Influence of Human p53 on Plant Development
Influence of Human p53 on Plant Development
复制标题
人类 p53 对植物发育的影响
DOI：
10.1371/journal.pone.0162840
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发表时间：
2016-09
期刊：
影响因子：
3.7
通讯作者：
Wang Shui
中科院分区：
文献类型：
--
作者：
Ma Huimin;Song Teng;Wang Tianhua;Wang Shui
哺乳动物的P53是一种超级肿瘤抑制因子，在保护基因组免受DNA损伤方面起着关键作用。然而，没有癌症的植物虽然经常暴露在紫外线的电离辐射下，但没有发现P53。在这里，我们将P53导入模式植物拟南芥，并检测了P53在植物中的表型。最引人注目的是，P53导致了早衰和筋膜的形成。在植物中，由于同源DNA重组的增加，已经显示出扁平化。一份带有GUS基因重叠片段(1445)的报道一致地表明，在转P53基因的植物中，同源重组的频率很高。与P53相反，NPR1-1诱导型抑制因子1(SNI1)作为植物同源重组的负调控因子，在哺乳动物中是不存在的。彗星实验和克隆存活实验表明，SNI1抑制电离辐射或羟基脲对人骨肉瘤U2OS癌细胞DNA损伤的修复作用。RAD51D是一种同源重组重组酶，作用于植物SNI1下游。有趣的是，P53使SNI1突变体的花序疯狂分枝，而RAD51D突变体完全抑制了P53诱导的表型，表明人类P53在植物中的作用是通过SNI1-RAD51D信号通路介导的。P53和SNI1在人和拟南芥中的物种互换试验表明，这些物种特异的蛋白在动植物王国的同源重组中发挥着共同的作用。
Mammalian p53 is a super tumor suppressor and plays a key role in guarding genome from DNA damage. However, p53 has not been found in plants which do not bear cancer although they constantly expose to ionizing radiation of ultraviolet light. Here we introduced p53 into the model plant Arabidopsis and examined p53-conferred phenotype in plant. Most strikingly, p53 caused early senescence and fasciation. In plants, fasciation has been shown as a result of the elevated homologous DNA recombination. Consistently, a reporter with overlapping segments of the GUS gene (1445) showed that the frequency of homologous recombination was highly induced in p53-transgenic plants. In contrast to p53, SUPPRESSOR OF NPR1-1 INDUCIBLE 1 (SNI1), as a negative regulator of homologous recombination in plants, is not present in mammals. Comet assay and clonogenic survival assay demonstrated that SNI1 inhibited DNA damage repair caused by either ionizing radiation or hydroxyurea in human osteosarcoma U2OS cancer cells. RAD51D is a recombinase in homologous recombination and functions downstream of SNI1 in plants. Interestingly, p53 rendered the sni1 mutants madly branching of inflorescence, a phenotype of fasciation, whereas rad51d mutant fully suppressed the p53-induced phenotype, indicating that human p53 action in plant is mediated by the SNI1-RAD51D signaling pathway. The reciprocal species-swap tests of p53 and SNI1 in human and Arabidopsis manifest that these species-specific proteins play a common role in homologous recombination across kingdoms of animals and plants.
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影响因子：
3.6
作者：
H. Stürzbecher;B. Donzelmann;S. Buchhop
通讯作者：
H. Stürzbecher;B. Donzelmann;S. Buchhop
影响因子：
3.8
作者：
Jensen, Ryan B.;Ozes, Ali;Kim, Taeho;Estep, Allison;Kowalczykowski, Stephen C.
通讯作者：
Kowalczykowski, Stephen C.
影响因子：
4.6
作者：
H. Leyser;I. Furner
通讯作者：
H. Leyser;I. Furner
DOI：
10.1002/j.1460-2075.1987.tb02730.x
发表时间：
1987-12
期刊：
The EMBO Journal
影响因子：
--
作者：
R. Jefferson;T. Kavanagh;M. Bevan
通讯作者：
R. Jefferson;T. Kavanagh;M. Bevan
影响因子：
21.3
作者：
Jazayeri, A;Falck, J;Jackson, SP
通讯作者：
Jackson, SP