Characterization of eight novel microsatellite markers for the neotropical bat Rhynchonycteris naso and cross-species utility
Characterization of eight novel microsatellite markers for the neotropical bat Rhynchonycteris naso and cross-species utility
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新热带蝙蝠 Rhynchonycteris naso 的八种新型微卫星标记的表征和跨物种实用性
DOI：
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发表时间：
2010
影响因子：
1.1
通讯作者：
F. Mayer
中科院分区：
文献类型：
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作者：
M. Nagy;Jana Ustinova;F. Mayer
众所周知，新热带蝙蝠在社会系统和扩散策略方面表现出异常的多样性。在这里，我们报告了八个新的微卫星标记的分离，这些标记将用于通过亲子关系、相关性和种群结构分析来调查性别偏见传播的最终原因和后果。为Rhynchonycteris naso物种开发的新型微卫星标记被证明具有高度多态性。每个位点的等位基因数量为 7 至 31 个等位基因，预期杂合度为 0.74 至 0.95。七个基因座是常染色体，一个基因座似乎位于 X 染色体上。四个物种的跨物种扩增在亲缘关系较近的新热带金龟属中比在亲缘关系较远的属中更成功。与最初为 Saccopteryx bilineata 分离的其他 11 颗微卫星一起，我们现在拥有用于研究 Saccopteryx 和 Rhynchonycteris 两个蝙蝠属物种的强大工具。
Neotropical emballonurid bats are known to exhibit an exceptional diversity in social systems and dispersal strategies. Here we report the isolation of eight new microsatellite markers which will be employed to investigate ultimate causes and consequences of sex-biased dispersal by use of paternity, relatedness and population structure analyses. Novel microsatellite markers developed for the species Rhynchonycteris naso turned out to be highly polymorphic. Number of alleles ranged from seven to 31 alleles per locus and expected heterozygosities ranged from 0.74 to 0.95. Seven loci were autosomal and one locus appeared to be situated on the X-Chromosome. Cross-species amplification in four species was more successful in more closely related than in more distantly related genera of neotropical emballonurids. Together with other eleven microsatellites initially isolated for Saccopteryx bilineata, we now posses powerful tools for studying species of the two bat genera, Saccopteryx and Rhynchonycteris.