Up-regulation of P2X7 receptors mediating proliferation of Schwann cells after sciatic nerve injury.
Up-regulation of P2X7 receptors mediating proliferation of Schwann cells after sciatic nerve injury.
复制标题
坐骨神经损伤后 P2X7 受体上调介导雪旺细胞增殖。
DOI：
10.1007/s11302-015-9445-8
复制
发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Xiang Zhenghua
中科院分区：
文献类型：
--
作者：
Song Xian-min;Xu Xiao-hui;Zhu Jiao;Guo Zhili;Li Jian;He Cheng;Burnstock Geoffrey;Yuan Hongbin;Xiang Zhenghua
周围神经损伤（Peripheral Nerve Injury，PNI）是一种常见的神经系统疾病，可导致运动、感觉和自主神经功能的部分或全部丧失，从而降低患者的生活质量。雪旺细胞在维持周围神经系统以及损伤和修复中发挥重要作用。应用免疫组化、Western blot、钙离子测定和BrdU增殖试验，本研究清楚地证明了P2 X7受体（R）在坐骨神经纵切面的有髓和无髓雪旺细胞中均有表达。坐骨神经损伤（SNI）后，雪旺细胞P2 X7 R表达在SNI早期明显上调。增殖细胞核抗原（PCNA）和P2 X7 R的双重免疫荧光显示，P2 X7 R可能参与了雪旺细胞的增殖。对原代培养的雪旺细胞的进一步实验表明，P2 X7 R在大鼠坐骨神经的雪旺细胞中功能性表达; ATP通过P2 X7 R可以促进雪旺细胞增殖，可能通过MAPK/ERK细胞内信号通路。P2 X7 R对雪旺细胞的其他可能的作用进行了讨论。
Peripheral nerve injury (PNI) is a common disease, which results in a partial or total loss of motor, sensory and autonomic functions, leading to a decrease in quality of life. Schwann cells play a vital role in maintaining the peripheral nervous system and in injury and repair. Using immunohistochemistry, Western blot, calcium assay and bromodeoxyuridine (BrdU) proliferation assay, the present study clearly demonstrated that P2X7 receptors (R) were expressed in myelinating and non-myelinating Schwann cells in longitudinal sections of sciatic nerves. After sciatic nerve injury (SNI), P2X7R expression in Schwann cells of injured sciatic nerves was significantly up-regulated during the early days of SNI. Double immunofluorescence of proliferating cell nuclear antigen (PCNA) and P2X7R implied that P2X7R may be involved in proliferation of Schwann cells. Further experiments on primary cultures of Schwann cells showed that P2X7R are functionally expressed in Schwann cells of rat sciatic nerves; ATP via P2X7R can promote Schwann cell proliferation, possibly via the MAPK/ERK intracellular signalling pathway. Other possible roles of P2X7R on Schwann cells are discussed.