Physical mapping of paar mutations of herpes simplex virus type 1 and type 2 by intertypic marker rescue
Physical mapping of paar mutations of herpes simplex virus type 1 and type 2 by intertypic marker rescue
复制标题
通过型间标记救援对 1 型和 2 型单纯疱疹病毒的 paar 突变进行物理定位
DOI：
10.1128/jvi.31.2.265-276.1979
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发表时间：
1979
影响因子：
5.4
通讯作者：
N. Wilkie
中科院分区：
文献类型：
--
作者：
P. Chartrand;N. Stow;M. Timbury;N. Wilkie
单纯疱疹病毒（HSV）中赋予膦酰乙酸抗性的突变（帕尔）涉及与病毒诱导的DNA聚合酶活性相关的基因。HSV的两种突变体（HSV-1 tsH和HSV-2 ts 6）产生热不稳定的DNA聚合酶活性。在这项研究中，TS病变存在于这些突变体和那些存在于两个独立的膦酰乙酸抗性HSV-1和HSV-2的突变体（帕尔-1和帕尔-2）已物理映射的限制性内切酶分析的重组体之间产生的HSV-1和HSV-2的型间标记拯救。所有四个突变都定位在图谱单元40周围的3.3-内切酶对区域内。tsH和帕尔-2的定位结果反映了该方法的准确性，发现它们位于相同的1.3-脱氢酶对区域。发现帕尔-1位于ts 6的右侧。病毒诱导的DNA聚合酶被认为具有150，000的分子量，需要具有4.6个酶对的编码能力的基因。这项研究中绘制的四个突变都位于比这个更小的区域内，但结果还不能证明所有四个病变都位于这个或任何一个基因中。
Mutations (paar) in herpes simplex virus (HSV) which confer resistance to phosphonoacetic acid involve genes associated with virus-induced DNA polymerase activity. Two mutants of HSV (HSV-1 tsH and HSV-2 ts6) produce a thermolabile DNA polymerase activity. In this study, the ts lesions present in these mutants and those present in two independent phosphonoacetic acid-resistant mutants of HSV-1 and HSV-2 (paar-1 and paar-2) have been physically mapped by restriction endonuclease analysis of recombinants produced between HSV-1 and HSV-2 by intertypic marker rescue. All four mutations mapped within a 3.3-kilobase pair region around map unit 40. The accuracy of the method is reflected by the mapping results for tsH and paar-2, which were found to lie in the same 1.3-kilobase pair region. paar-1 was found to lie to the right of ts6. Virus-induced DNA polymerase is thought to have a molecular weight of 150,000, necessitating a gene with a coding capacity of 4.6 kilobase pairs. The four mutations mapped in this study all lie within a region smaller than this, but the results do not yet prove that all four lesions reside in this or any single gene.