Influence of histone H1 on chromatin structure.
Influence of histone H1 on chromatin structure.
复制标题
组蛋白 H1 对染色质结构的影响。
DOI：
10.1016/0006-291x(80)91264-4
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发表时间：
1980
影响因子：
3.1
通讯作者：
Bernard Roux
中科院分区：
文献类型：
--
作者：
C. Marion;Bernard Roux
通过研究瞬时电双折射（TEB）来研究组蛋白H1对寡核体构象的影响。整个染色质呈现正双折射，这可以用螺旋紧凑结构来解释，其中DNA的超螺旋轴平行于螺旋阵列的长轴。缺失H1的寡核小体的双链构象为负，并与链中核小体的数目成正比，因此，去除H1后，寡核小体转变为一种延伸的“珠桥”结构，其中32个碱基对的DNA连接两个连续的珠。然后提出了以下DNA方案：195 bp（大鼠肝脏中的核小体重复序列）= 163 bp（与组蛋白核心相关）+32 bp（接头DNA）。
The influence of histone H1 with respect to the conformation of oligonucleosomes was investigated by studying transient electric birefringence (TEB). Whole chromatin exhibits a positive birefringence explained by an helical compact structure in which the superhelical axis of DNA is parallel to the long axis of the helical array. Birefringence of H1-lacking oligonucleosomes is negative and proportional to the number of nucleosomes in the chain.So, the removal of H1 induces a transition to an extended “bead and bridge” structure in which 32 base-pairs of DNA connect two consecutive beads. The following scheme is then proposed for DNA: 195 bp (nucleosome repeat in rat liver) = 163 bp (associated with the histone core) + 32 bp (linker DNA).