Nucleolar organizer regions in hepatocarcinogenesis induced by N-2-fluorenylacetamide in rats: comparison with bromodeoxyuridine immunohistochemistry.
Nucleolar organizer regions in hepatocarcinogenesis induced by N-2-fluorenylacetamide in rats: comparison with bromodeoxyuridine immunohistochemistry.
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DOI：
10.1111/j.1349-7006.1989.tb02257.x
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发表时间：
1989-11
期刊：
影响因子：
--
通讯作者：
Mori H
中科院分区：
文献类型：
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作者：
Tanaka T;Takeuchi T;Nishikawa A;Takami T;Mori H
用银染核仁蛋白(AgNOR)对N-2-荧乙酰胺(FAA)诱导的大鼠肝脏癌前病变和肿瘤性病变进行了计数，并与用抗BrdU单抗免疫组织化学方法检测的掺入BrdU的细胞进行了比较。雄性ACI/N大鼠喂饲含200ppm FAA的饲料12、16、20周，诱发肝细胞瘤灶形成。银胶体一步法染色及BrdU标记指数分别为：未治疗组20例，1.20例，0.08例，非病变区20例，1.33例，0.13例，变灶80例，2.04例，4.05例，嗜酸性细胞型20例，1.78例，1.82例，透明细胞型20例，1.45例，1.77例，嗜碱性细胞型20例，1.99例，4.58例；嗜碱性细胞型20例，分别为2.94和8.02；肿瘤结节10例，分别为3.11和2.99；肝细胞癌10例，分别为7.22和8.29。因此，AgNOR的平均数与BrdU标记指数的值有很好的相关性，两者的值都显示出从正常肝细胞到肝细胞癌的逐步增加，尽管存在一些分散。这些结果提示，AgNOR的平均数量可能反映了大鼠肝癌发生过程中的细胞动力学，一步法银胶显示AgNOR可能是一种简单而有用的染色方法，可用于检测细胞的增殖性质。
The number of silver‐stained nucleolar proteins (AgNOR) was counted in preneoplastic and neoplastic rat liver lesions induced by N‐2‐fluorenylacetamide (FAA) and was compared with that of bromodeoxyuridine (BrdU)‐incorporating cells detected immunohistochemically using monoclonal antibody against BrdU. Male ACI/N rats were given diet containing 200 ppm FAA for 12, 16 or 20 weeks to induce hepatocellular foci and tumors. The mean numbers of AgNOR stained by a one‐step silver colloid method and BrdU‐labeling indices in various liver cell lesions were as follows: nontreated liver (n = 20), 1.20 and 0.08; nonlesional areas (n=20), 1.33 and 0.13; altered liver cell foci (n = 80), 2.04 and 4.05 [eosinophilic cell type (n = 20), 1.78 and 1.82; clear cell type (n=20), 1.45 and 1.77; basophilic cell type (n=20), 1.99 and 4.58; hyperbasophilic cell type (n=20), 2.94 and 8.02]; neoplastic nodules (n = 10), 3.11 and 2.99; hepatocellular carcinomas (n = 10), 7.22 and 8.29. Thus, the mean number of AgNOR and the value of BrdU‐labeling index were well correlated and both values showed a stepwise increase from normal liver cells to liver cell carcinoma, although some scatter was present. These data suggest that mean number of AgNOR may reflect the cellular kinetics in rat hepatocarcinogenesis, and the one‐step silver colloid method for demonstration of AgNOR may therefore be a simple and useful staining to examine the proliferative nature of cells.