Serologic and molecular detection of Ehrlichia chaffeensis and Anaplasma phagocytophila (human granulocytic ehrlichiosis agent) in Korean patients
Serologic and molecular detection of Ehrlichia chaffeensis and Anaplasma phagocytophila (human granulocytic ehrlichiosis agent) in Korean patients
复制标题
DOI：
10.1128/jcm.40.8.3082-3085.2002
复制
发表时间：
2002-08-01
影响因子：
9.4
通讯作者：
Chae, JS
中科院分区：
文献类型：
--
作者：
Heo, EJ;Park, JH;Chae, JS
采用间接免疫荧光法（IFA）、免疫印迹法（Western blotting）和TaqMan实时聚合酶链反应（real - time PCR）检测了491例韩国急性发热性疾病患者血清中恰菲埃利体和嗜吞噬细胞无原体抗体。总体而言，在IFAs中，0.4%的血清与沙菲奈瑟菌反应，1.8%与嗜吞噬细胞奈瑟菌反应。这是国内首次检测到嗜吞噬单胞虫和沙非伊虫的抗体，提示存在嗜吞噬单胞虫和沙非伊虫或抗原性相似的种。
Sera from 491 Korean patients with acute febrile diseases were tested for Ehrlichia chaffeensis and Anaplasma phagocytophila antibodies by indirect immunofluorescence assay (IFA), Western blotting, and TaqMan realtime PCR. Overall, 0.4% of sera reacted with E. chaffeensis, and 1.8% reacted with A. phagocytophila in IFAs. This is the first report of detection of antibodies to A. phagocytophila and E. chaffeensis in Korea and suggests the presence of A. phagocytophila and E. chaffeensis or antigenically similar species.