4-METHYLUMBELLIFERYL 2-ACETAMIDO-2-DEOXY-ALPHA-D-GLUCOPYRANOSIDE, A FLUOROGENIC SUBSTRATE FOR N-ACETYL-ALPHA-D-GLUCOSAMINIDASE
4-METHYLUMBELLIFERYL 2-ACETAMIDO-2-DEOXY-ALPHA-D-GLUCOPYRANOSIDE, A FLUOROGENIC SUBSTRATE FOR N-ACETYL-ALPHA-D-GLUCOSAMINIDASE
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DOI：
10.1016/s0008-6215(00)84698-4
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发表时间：
1981-01-01
影响因子：
3.1
通讯作者：
WEISSMANN, B
中科院分区：
文献类型：
--
作者：
CHOW, P;WEISSMANN, B
Condensation of dimeric 3,4,6-tri-O-acetyl-2-deoxy-2-nitroso-.alpha.-D-glucopyranosyl chloride with 4-methylumbelliferone gave crystalline 4-methylumbelliferyl 3,4,6-tri-O-acetyl-2-deoxy-2-oximino-.alpha.-D-arabino-hexopyranoside. Acetylation of this adduct, reduction of the resulting crude O-acetyloxime with borane in oxolane, and acetylation gave the 3,4,6-tri-O-acetyl derivative of 4-methylumbelliferyl 2-acetamido-2-deoxy-.alpha.-D-glucopyranoside (1). A new sensitive assay of N-acetyl-.alpha.-D-glucosaminidase is made possible by fluorometric measurement of 4-methylumbelliferone liberated by enzymic hydrolysis of glycoside 1. Such assays are illustrated by results obtained with enzyme preparations from pig liver and human blood serum.
