Genome-Wide Imaging-Based Phenomic Screening Using Yeast (Saccharomyces cerevisiae) Strain Collections.
Genome-Wide Imaging-Based Phenomic Screening Using Yeast (Saccharomyces cerevisiae) Strain Collections.
复制标题
使用酵母（酿酒酵母）菌株进行基于全基因组成像的表型筛选。
DOI：
10.1007/978-1-0716-0868-5_8
复制
发表时间：
2021
影响因子：
--
通讯作者：
Beidong Liu
中科院分区：
文献类型：
--
作者：
Xiuling Cao;Xuejiao Jin;Beidong Liu
表型研究可以提供一个系统的概述表型，基因型和环境因素之间的相互作用网络。酵母（酿酒酵母）是表型研究中最重要的模式生物之一，因为有大量的全基因组菌株。我们描述了一个详细的协议进行酵母表型筛选的蛋白质共定位的评价通过全基因组成像为基础的筛选方法，利用GFP标记的酵母菌株收集。
Phenomic studies can provide a systemic overview of the network of interactions between phenotypes, genotypes, and environmental factors. Yeast (Saccharomyces cerevisiae) is one of the most important model organisms for phenomic studies due to the availability of a large variety of genome-wide strain collections. We describe a detailed protocol for performing a yeast phenomic screen for evaluation of protein colocalization via a genome-wide imaging-based screening approach utilizing a GFP-tagged yeast strain collection.
影响因子：
16
作者：
Sopko, R;Huang, DQ;Andrews, B
通讯作者：
Andrews, B