NeuroGT: A brain atlas of neurogenic tagging CreER drivers for birthdate-based classification and manipulation of mouse neurons.
NeuroGT: A brain atlas of neurogenic tagging CreER drivers for birthdate-based classification and manipulation of mouse neurons.
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DOI：
10.1016/j.crmeth.2021.100012
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发表时间：
2021-07-26
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神经元出生日期是神经元表型的主要决定因素之一。然而，大多数出生日期的方法是回顾性的，允许很少的实验访问分类神经元子集。在这里，我们介绍了四个神经源性标记小鼠系，它可以分配CreER-loxP重组的神经元子集，共享相同的分化时间在活的动物，并使各种实验操作的分类子集。我们构建了神经原性标记小鼠品系（NeuroGT）的大脑图谱，其中包括在神经发育期间每天标记的loxP重组神经元及其整个大脑过程的整体图像数据。该图像数据库向公众开放，为研究人员提供了找到特定神经源性标记驱动线和适合他们自己研究目的的标记阶段的机会。我们生成了四个CreER小鼠驱动程序，以根据神经发生的时间标记神经元。驱动程序可以对神经元进行基于出生日期的分类和操作。我们开发了NeuroGT，这是整个大脑中标记神经元的图像图谱。通过NeuroGT，研究人员可以找到适合自己研究的驱动程序线。神经元出生日期通常用于对神经元进行分类。然而，目前基于核苷酸的出生日期方法本质上是回顾性的，并且不允许操纵分类的神经元。为了克服这一限制，我们开发了神经源性标记小鼠系，可以诱导CreER-loxP重组神经元具有相似的出生日期，重组标签可以随后用于实验操作的分类神经元子集。为了鼓励使用这种资源，我们启动了NeuroGT数据库，其中包含整个大脑中标记神经元的图像数据。Hirata等人开发神经源性标记CreER驱动小鼠和NeuroGT数据库以展示小鼠品系。该资源可用于根据神经元的生成时间对其进行分类，并出于研究目的操纵分类的神经元子集。
Neuronal birthdate is one of the major determinants of neuronal phenotypes. However, most birthdating methods are retrospective in nature, allowing very little experimental access to the classified neuronal subsets. Here, we introduce four neurogenic tagging mouse lines, which can assign CreER-loxP recombination to neuron subsets that share the same differentiation timing in living animals and enable various experimental manipulations of the classified subsets. We constructed a brain atlas of the neurogenic tagging mouse lines (NeuroGT), which includes holistic image data of the loxP-recombined neurons and their processes across the entire brain that were tagged on each single day during the neurodevelopmental period. This image database, which is open to the public, offers investigators the opportunity to find specific neurogenic tagging driver lines and the stages of tagging appropriate for their own research purposes. We generate four CreER mouse drivers to tag neurons on the basis of time of neurogenesis The drivers enable birthdate-based classification and manipulation of neurons We develop NeuroGT, an image atlas of tagged neurons across the entire brain Through NeuroGT, researchers can find driver lines suitable for their own research Neuronal birthdates are commonly used to classify neurons. However, the current nucleotide-based birthdating method is retrospective in nature and does not allow the manipulation of classified neurons. To overcome this limitation, we developed neurogenic tagging mouse lines that can induce CreER-loxP recombination in neurons with similar birthdates, and the recombination tag can be subsequently used for experimental manipulation of the classified neuron subsets. To encourage the use of this resource, we launched the NeuroGT database, which contains image data of the tagged neurons across the entire brain. Hirata et al. develop neurogenic tagging CreER driver mice and the NeuroGT database to showcase the mouse lines. The resource can be used to classify neurons on the basis of their generation timing and manipulate the classified neuron subsets for research purposes.
影响因子：
2.9
作者：
Szabó NE;Haddad-Tóvolli R;Zhou X;Alvarez-Bolado G
通讯作者：
Alvarez-Bolado G