Bacteriocins: mechanism of membrane insertion and pore formation
Bacteriocins: mechanism of membrane insertion and pore formation
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DOI：
10.1023/a:1002002718501
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发表时间：
1999-11-01
影响因子：
2.6
通讯作者：
Driessen, AJM
中科院分区：
文献类型：
--
作者：
Moll, GN;Konings, WN;Driessen, AJM
乳酸菌产生几种类型的造孔肽。I类细菌素是含有(甲基)羊毛硫氨酸残基的抗生素，这些残基可能形成分子内的硫醚环。这些多肽通常具有广泛的活性，并形成不稳定的毛孔。第二类细菌素是一种小的、耐热的多肽，其活性谱很窄。大多数细菌素与革兰氏阳性细菌细胞膜中大量存在的阴离子脂类相互作用。“对接分子”可以增强抗生素毛孔的导电性和稳定性，而靶膜上的“受体”可能决定第二类细菌素的特异性。许多细菌素的插入是由质子驱动力驱动的。抗生素可按“楔形”模型形成孔，而II类细菌素可通过形成“桶形壁”孔或“地毯”机制来增强膜的通透性。
Lactic acid bacteria produce several types of pore forming peptides. Class I bacteriocins are lantibiotics that contain (methyl)lanthionine residues that may form intramolecular thioether rings. These peptides generally have a broad spectrum of activity and form unstable pores. Class II bacteriocins are small, heat stable peptides mostly with a narrow spectrum of activity. Most bacteriocins interact with anionic lipids that are abundantly present in the membranes of Gram-positive bacteria. 'Docking molecules' may enhance the conductivity and stability of lantibiotic pores, while 'receptors' in the target membrane may determine specificity of class II bacteriocins. Insertion into the membrane of many bacteriocins is proton motive force driven. Lantibiotics may form pores according to a 'wedge-like' model, while class II bacteriocins may enhance membrane permeability either by the formation of a `barrel stave' pore or by a 'carpet' mechanism.