Glow discharge plasma pretreatment enhances osteoclast differentiation and survival on titanium plates
Glow discharge plasma pretreatment enhances osteoclast differentiation and survival on titanium plates
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DOI：
10.1016/j.biomaterials.2003.08.032
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发表时间：
2004-05-01
期刊：
影响因子：
14
通讯作者：
Miyazaki, T
中科院分区：
文献类型：
--
作者：
Kawai, H;Shibata, Y;Miyazaki, T
尽管已有一些报道表明破骨细胞在各种生物活性陶瓷上具有活性，但表面修饰的钛上的破骨细胞功能还没有受到关注。本研究旨在检测参与含RGD(Arg-Gly-Asp)序列的蛋白质黏附的辉光放电等离子体(GDP)表面能的增加是否影响钛板的破骨细胞反应。我们研究了在有无GDP的钛板上破骨细胞分化和存活率。1周后，GDP对钛板上破骨细胞数量无明显影响。然而，经GDP处理后，破骨细胞分化被大大激活，因为经GDP处理的钛板上抗酒石酸酸性磷酸酶的合成显著增加。此外，由于破骨细胞仅在含GDP的钛板上检测到，即使在共培养实验中培养4h后，经GDP预处理的破骨细胞存活率也有所提高。由于破骨细胞的反应即使在表面修饰的金属材料上也会受到影响，我们得出结论，不仅需要在陶瓷材料上进行破骨细胞的吸收，也需要在表面修饰的金属材料上进行破骨细胞反应。(C)2003爱思唯尔有限公司。保留所有权利。
Despite the fact that several reports have demonstrated osteoclast activity on various bioactive ceramics, osteoclast functions on surface-modified titanium have not come under focus. This study aimed to examine whether the increasing surface energy of glow discharge plasma (GDP) involved in protein adhesion containing the RGD (Arg-Gly-Asp) sequence affects osteoclast responses oil titanium plates. We examined osteoclast differentiation and survival rates on titanium plates with and without GDP. The amounts of osteoclasts on titanium plates were not increased by GDP after 1 week. However, osteoclast differentiation was greatly activated by GDP pretreatment, as tartrate-resistant acid phosphatase synthesis significantly increased on the titanium plates with GDP. Additionally, since the presence of osteoclasts was detected only on the titanium plates with GDP, even after 4 h cultivation in a coculture test, the osteoclasts survival rate was increased by GDP pretreatment. As osteoclast responses were affected even on surface modified metallic materials, we concluded that novel approaches are needed not only for osteoclastic resorption on ceramic materials but also for osteoclast responses on surface-modified metallic materials. (C) 2003 Elsevier Ltd. All rights reserved.