Similar to Those Who Are Breastfed, Infants Fed a Formula Containing 2′-Fucosyllactose Have Lower Inflammatory Cytokines in a Randomized Controlled Trial
Similar to Those Who Are Breastfed, Infants Fed a Formula Containing 2′-Fucosyllactose Have Lower Inflammatory Cytokines in a Randomized Controlled Trial
复制标题
DOI：
10.3945/jn.116.236919
复制
发表时间：
2016-12-01
影响因子：
4.2
通讯作者：
Buck, Rachael H.
中科院分区：
文献类型：
--
作者：
Goehring, Karen C.;Marriage, Barbara J.;Buck, Rachael H.
背景：有证据表明，母乳低聚糖（HMOs）对婴儿有多种益处，包括益生元效应、肠道成熟、抗菌活性和免疫调节。需要对HMOs进行临床干预研究，以证实婴儿的这些益处。目的：我们的目的是研究添加HMO 2'-聚焦乳糖（2'- fl）的喂养配方对健康足月婴儿免疫功能生物标志物的影响。方法：我们在一项随机、双盲、对照生长和耐受性研究中进行了一项亚研究，研究对象是健康的单胎婴儿（出生体重b> = 2490 g），从出生第5天开始入组，从入组到4个月大，全部采用配方奶喂养（n = 317）或母乳喂养（n = 107）。配方奶粉喂养的婴儿被随机分配接受3种配方奶粉中的1种，这些配方奶粉的总低聚糖含量均为2.4 g /L[对照组：仅含半乳糖低聚糖（GOS）；实验配方：GOS + 0.2或1.0 g 2′-FL/L]，并与母乳喂养参照组进行比较。在本亚研究中，从6周龄的婴儿中抽取血液样本（n = 31-42/组）。分离外周血单个核细胞（PBMCs）进行细胞表型分析，并在体外用植物血凝素刺激细胞增殖和细胞周期进程或呼吸道合胞病毒（RSV）。测定血浆和体外刺激培养上清液中细胞因子的浓度。结果：母乳喂养的婴儿和饲喂2′-FL的实验配方奶的婴儿血浆炎症因子浓度没有差异，但比饲喂对照配方奶的婴儿血浆炎症因子浓度[白细胞介素（IL）受体拮抗剂（IL-1ra）、IL-1 α、IL-1 β、IL-6和肿瘤坏死因子α (tnf - α)]低29-83% (P
Background: Evidence suggests that human milk oligosaccharides (HMOs) provide multiple benefits to infants, including prebiotic effects, gut maturation, antimicrobial activities, and immune modulation. Clinical intervention studies with HMOs are required to confirm these benefits in infants.Objective: Our objective was to investigate the effects of feeding formulas supplemented with the HMO 2'-fucosyllactose (2'-FL) on biomarkers of immune function in healthy term infants.Methods: We performed a substudy nested within a randomized, double-blind, controlled growth and tolerance study in healthy singleton infants (birth weight >= 2490 g) who were enrolled by 5 d of life and exclusively formula-fed (n = 317) or breastfed (n = 107) from enrollment to 4 mo of age. Formula-fed infants were randomly assigned to receive 1 of 3 formulas, all containing 2.4 g total oligosaccharides/L [control: galacto-oligosaccharides (GOS) only; experimental formulas: GOS + 0.2 or 1.0 g 2'-FL/L], and compared with a breastfed reference group. For this substudy, blood samples were drawn from infants at 6 wk of age (n = 31-42/group). Peripheral blood mononuclear cells (PBMCs) were isolated for cellular phenotyping and stimulated ex vivo with phytohemagglutinin for proliferation and cell cycle progression or respiratory syncytial virus (RSV). Cytokine concentrations were measured in plasma and in ex vivo-stimulated culture supernatants.Results: Breastfed infants and infants fed either of the experimental formulas with 2'-FL were not different but had 29-83% lower concentrations of plasma inflammatory cytokines than did infants fed the control formula [interleukin (IL) receptor antagonist (IL-1ra), IL-1 alpha, IL-1 beta, IL-6, and tumor necrosis factor alpha (TNF-alpha)] (P