Heterologous priming-boosting with DNA and modified vaccinia virus Ankara expressing tryparedoxin peroxidase promotes long-term memory against Leishmania major in susceptible BALB/c Mice.
Heterologous priming-boosting with DNA and modified vaccinia virus Ankara expressing tryparedoxin peroxidase promotes long-term memory against Leishmania major in susceptible BALB/c Mice.
复制标题
用 DNA 和表达胰蛋白酶过氧化物酶的修饰痘苗病毒安卡拉进行异源启动增强可促进易感 BALB/c 小鼠对抗重大利什曼原虫的长期记忆。
DOI：
10.1128/iai.01490-06
复制
发表时间：
2007
影响因子：
3.1
通讯作者：
Blackwell,JeneferM
中科院分区：
文献类型：
--
作者：
Stober,CarmelB;Lange,UtaG;Roberts,MarkTM;Alcami,Antonio;Blackwell,JeneferM
Leishmaniasis affects 12 million people, but there are no vaccines in routine clinical use. Th1 polarizing vaccines that elicit long-term protection are required to prevent disease in susceptible populations. We recently showed that heterologous priming-boosting with tryparedoxin peroxidase (TRYP) DNA followed by TRYP-modified vaccinia virus Ankara (TRYP MVA) protected susceptible BALB/c mice fromLeishmania major. Here we compared treatment with TRYP DNA with treatment with TRYP DNA/TRYP MVA. We found that equivalent levels of protection during the postvaccination effector phase correlated with equivalent levels of serum immunoglobulin G2a and gamma interferon (IFN-γ) in draining lymph nodes. In contrast, challenge infection during the memory phase revealed that there was enhanced clinical efficacy with TRYP DNA/TRYP MVA. This correlated with higher levels of effector phase splenic IFN-γ, sustained prechallenge levels of memory phase IFN-γ, and a more polarized post-L. majorchallenge Th1 response compared to the Th2/Tregresponse. Thus, TRYP DNA/TRYP MVA, but not TRYP DNA alone, provides long-term protection against murine leishmaniasis.
