SARS‐CoV ‐2 spike protein capture by peptide functionalized networks
SARS‐CoV ‐2 spike protein capture by peptide functionalized networks
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DOI：
10.1002/pol.20220539
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发表时间：
2022-11
影响因子：
3.4
通讯作者：
M. Rahman;Chamoni W. H. Rajawasam;Nethmi De Alwis Watuthanthrige;J. L. Sparks;R. Page;Dominik Konkolewicz-Dominik-Konkol
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文献类型：
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作者：
M. Rahman;Chamoni W. H. Rajawasam;Nethmi De Alwis Watuthanthrige;J. L. Sparks;R. Page;Dominik Konkolewicz-Dominik-Konkol
2019冠状病毒病（COVID-19）对人类健康、全球经济和社会产生了重大影响。驻留在公共表面上的病毒是一般人群的潜在污染源。刺突结合肽1，SBP 1是一种23个氨基酸的肽，其对刺突蛋白受体结合结构域具有微摩尔结合亲和力（1.3 μ M）。我们假设，如果我们可以在共价交联网络系统中共价地连接这种SBP 1肽，我们可以开发出一种优先结合刺突蛋白的表面，因此可以限制病毒的传播。制备了一系列具有不同主链长度和交联剂密度的丙烯酸羟乙酯（HEA）共价交联网络。随后，使用2%SBP 1肽将该网络系统功能化。我们的研究发现，在较短的链长和较低的交联剂密度下，单独的HEA网络系统可以捕获几乎80%的刺突蛋白。我们报道，效率可以提高近17%，与更高的交联剂密度。
Coronavirus disease 2019 (COVID-19) has significantly impacted human health, the global economy, and society. Viruses residing on common surfaces represent a potential source of contamination for the general population. Spike binding peptide 1, SBP1 is a 23 amino acid peptide, which has micromolar binding affinity (1.3 mu M) towards the spike protein receptor-binding domain. We hypothesize that if we can covalently immobilize this SBP1 peptide in a covalent crosslinked network system, we can develop a surface that would preferentially bind spike protein and, therefore, which could limit viral spread. A series of covalently crosslinked networks of hydroxy ethyl acrylate (HEA) with different primary chain lengths and crosslinker density was prepared. Later, this network system was functionalized using 2% SBP1 peptide. Our study found that with a shorter chain length and lower crosslinker density, the HEA network system alone could capture almost 80% of the spike protein. We reported that the efficiency could be enhanced almost by 17% with higher crosslinker density.