Escherichia coli O157:H7 in faeces from cattle, sheep and pigs in the southwest part of Norway during 1998 and 1999
Escherichia coli O157:H7 in faeces from cattle, sheep and pigs in the southwest part of Norway during 1998 and 1999
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DOI：
10.1016/s0168-1605(00)00518-3
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发表时间：
2001-05-10
影响因子：
5.4
通讯作者：
Herikstad, H
中科院分区：
文献类型：
--
作者：
Johnsen, G;Wasteson, Y;Herikstad, H
从1998年1月到1999年12月的两年期间，使用免疫磁性分离程序对1541头牛、665只羊和1976头猪的肠道内容物进行了大肠杆菌O157:H7分析。这些动物分别来自挪威西南部的 848、605 和 832 个畜群。大肠杆菌 O157:H7 存在于来自不同牛群的三个牛样本中，牛群患病率为 0.35%，动物患病率为 0.19%。从猪中，从不同猪群的两只猪中分离出大肠杆菌 O157:H7，猪群患病率为 0.24%，动物患病率为 0.1%。一项后续研究显示，其中一个猪群中的另一头猪检测结果呈阳性。在 665 只被调查的羊中，没有发现大肠杆菌 O157:H7。通过 PCR 分析，所有 6 株大肠杆菌 O157:H7 分离株均含有编码志贺毒素 2 (stx(2))、intimin 蛋白 (eae) 和 H7 鞭毛 (fliC-H7) 的基因。其中一种牛分离株还含有志贺毒素 1 编码 (6,) 基因。这六种分离株被区分为三种脉冲场凝胶电泳图谱。结果表明，与其他欧洲国家相比，挪威西南部的牛、羊和猪中大肠杆菌 O157:H7 的出现率较低。 (C) 2001 Elsevier Science B.V，保留所有权利。
During a 2-year period from January 1998 to December 1999, intestinal content from 1541 cattle, 665 sheep and 1976 pigs were analysed for Escherichia coli O157:H7 using the immunomagnetic separation procedure. The animals originated from 848, 605 and 832 herds from the southwest part of Norway, respectively.E. coli O157:H7 was present in three samples from cattle from different herds, giving a herd prevalence of 0.35% and an animal prevalence of 0.19%. From pigs, E. coli O157:H7 was isolated from two pigs from different herds, giving a herd prevalence of 0.24% and an animal prevalence of 0.1%. A follow-up study revealed another positive testing pig from one of these herds. E. coli O157:H7 was not found from any of the 665 investigated sheep.By PCR analysis, all six E. coli O157:H7 isolates were shown to contain the genes encoding Shiga toxin 2 (stx(2)), the intimin protein (eae) and the H7 flagellum (fliC-H7). One of the cattle isolates also harboured the Shiga toxin 1 encoding (six,) gene. The six isolates were differentiated into three pulse-field gel electrophoresis profiles. The results indicate that the occurrence of a coli O157:H7 in cattle, sheep and pigs in the southwest part of Norway is low compared to other European countries. (C) 2001 Elsevier Science B.V, All rights reserved.