T1243, an alternative transcript of the mitochondrial T gene in Brassica juncea var. tumida, causes pollen abortion in Arabidopsis thaliana
T1243, an alternative transcript of the mitochondrial T gene in Brassica juncea var. tumida, causes pollen abortion in Arabidopsis thaliana
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T1243，芥菜线粒体 T 基因的替代转录物。
DOI：
10.1016/j.plantsci.2008.07.015
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发表时间：
2008
期刊：
影响因子：
5.2
通讯作者：
L. Xiaohui
中科院分区：
文献类型：
--
作者：
Y. Pei;Qiao Zengjie;C. Xuejun;C. Zhu;C. Jia;Yu Xin;Mai Lihua;L. Xiaohui
早期的报道揭示了茎瘤芥（Brassica juncea var. tumida）是一个与细胞质雄性不育（CMS）相关的基因。T基因的两个替代转录本T1170和T1243在不同发育阶段表现出不同的表达模式。在这项工作中，这两个转录本的潜在作用进行了研究，在拟南芥通过下的花椰菜花叶病毒（CaMV）35S启动子的控制下过表达。过表达T1243转录本的转基因植物产生的花药似乎没有花粉和几乎完全塌陷的角果，显示出严重的雄性不育表型，而过表达T1170的转基因株系是正常的。为了证实上述结果，我们在A. thaliana，T1243以这些株系为受体进行过表达。在T1243敲除系中花粉正常。在表达T1243的对照野生型品系中，植物显示花粉败育。我们观察到不育表型和T1243的表达水平之间呈正相关。通过用野生型花粉授粉T1243转基因株系进行测交。约半数后代表现花粉败育，表明T基因转录本T1243可引起雄性不育表型。
An earlier report revealed that the mitochondrial T gene of tumorous stem mustard (Brassica juncea var. tumida) is a cytoplasmic male sterility (CMS)-related gene. Two alternative transcripts of the T gene, T1170 and T1243, showed different expression patterns at different developmental stages. In this work, the potential roles of these two transcripts were studied in Arabidopsis thaliana through over-expression under the control of the cauliflower mosaic virus (CaMV) 35S promoter. Transgenic plants over-expressing the T1243 transcript produced anthers that appeared devoid of pollen and almost fully collapsed siliques, showing a severe male sterility phenotype, while transgenic lines over-expressing T1170 were normal. To confirm the above results, we constructed T1243-RNAi lines in A. thaliana, and T1243 was over-expressed using these lines as recipients. Pollen was normal in the T1243 knocked-out lines. In the control wild type lines expressing T1243, plants showed pollen abortion. We observed a positive correlation between the sterility phenotypes and the expression levels of T1243. Test-crossing was done by pollinating T1243 transgenic lines with wild type pollen. About half of the progeny showed pollen abortion, indicating that T1243, one of the T gene transcripts, can cause a male sterility phenotype.
II 组内含子的催化核心一瞥。
DOI：
10.1016/s0969-2126(02)00756-6
发表时间：
2002
期刊：
Structure (London, England : 1993)
影响因子：
--
作者：
Query,CharlesC
通讯作者：
Query,CharlesC