Parvovirus B19 genome as a single, two-state replicative and transcriptional unit
Parvovirus B19 genome as a single, two-state replicative and transcriptional unit
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DOI：
10.1016/j.virol.2005.12.014
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发表时间：
2006-04-10
期刊：
影响因子：
3.7
通讯作者：
Gallinella, G
中科院分区：
文献类型：
--
作者：
Bonvicini, F;Filippone, C;Gallinella, G
通过定量实时PCR和RT-PCR测定分析了细小病毒B19 DNA和不同类别RNA在允许和非允许感染细胞中的量的变化。在允许的骨髓单个核细胞、UT 7/Epo和KU 812 Ep 6细胞中，病毒DNA通常在48 hpi内增加，相对于输入DNA很少超过2 μ g。病毒RNA总是在2-6 hpi内存在，其增加超过病毒DNA的增加，直到36-49 hpi，并且在整个感染周期中，所有不同种类的病毒RNA都以稳定的相对量持续存在。在非允许的TF-1细胞中，病毒DNA没有增加，仅检测到一种最具代表性的单一类别的病毒RNA。我们的数据不支持B19病毒复制和转录的当前模型，包括在不同的早期和晚期表达模式，但提出了一种替代模型，表明B19病毒基因组应被视为一个单一的，两个状态的复制和转录单位。(c)2005年爱思唯尔公司All rights reserved.
The variation in the amount of parvovirus B19 DNA and different classes of RNA in permissive and non-permissive infected cells was analysed by means of quantitative real-time PCR and RT-PCR assays. In the permissive bone marrow mononuclear cells, UT7/Epo and KU812Ep6 cells, viral DNA usually increased within 48 hpi, rarely exceeding 2 Logs with respect to input DNA. Viral RNA was always present within 2-6 hpi, its increase paralleled that of viral DNA up to 36-49 hpi, and all the different classes of viral RNA were constantly represented in stable relative amounts throughout the infection cycle. In the non-permissive TF-1 cells, viral DNA did not increase and only one most represented single class of viral RNA was detected. Our data do not support the current model for B19 virus replication and transcription, consisting in different early and late expression patterns, but suggest an alternative model, indicating that the B19 virus genome should be considered a single, two-state replicative and transcriptional unit. (c) 2005 Elsevier Inc. All rights reserved.