Biodeinking of flexographic inks by fungal laccases using synthetic and natural mediators
Biodeinking of flexographic inks by fungal laccases using synthetic and natural mediators
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DOI：
10.1016/j.bej.2012.05.010
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发表时间：
2012-08-15
影响因子：
3.9
通讯作者：
Jesus Martinez, Maria
中科院分区：
文献类型：
--
作者：
Fillat, Ursula;Prieto, Alicia;Jesus Martinez, Maria
基于柔性版油墨的新印刷技术的使用阻碍了纸张回收过程中的油墨消除，因此有必要开发替代方法。用真菌漆酶对4种柔性版油墨的脱色进行了研究，其中3种来自担子菌(丛毛栓菌、刚冠鞘和球孢霉)，1种来自子囊菌嗜热菌，在人工和天然介质存在下，3种担子菌漆酶对柔性版油墨的脱色能力高于嗜热链霉菌。担子菌漆酶在没有介体的情况下，在长时间的反应中使油墨脱色，尽管天然或合成介体(尤其是HBT)的存在加速了这一过程。另一方面，嗜热链霉漆酶不能对所检测的墨水脱色。除了几乎完全脱色的R48墨水外，紫丁香型介质的加入导致了中等程度的脱色。在处理的第一个小时，当所有担子菌漆酶在介质存在的情况下都完全活跃时，脱色率最高。与其他担子菌漆酶在HBT存在下24 h后失活不同，球形葡萄球菌的酶即使在48 h后也不会被该介体灭活，而仅有的两种能促进该酶对油墨脱色的介体--乙酰丁香酮和丁香酸甲酯在短时间内完全失去了嗜热支原体漆酶的活性。(C)2012爱思唯尔B.V.保留所有权利。
The use of new printing technologies based on flexographic inks hampers ink elimination during paper recycling, making necessary the development of alternative methods. Decolorization of four flexographic inks has been evaluated by using fungal laccases, three of them from basidiomycetes (Trametes villosa, Coriolopsis rigida, and Pycnoporus coccineus), and one from the ascomycete Myceliophthora thermophila in the presence of synthetic and natural mediators.The results obtained showed a higher capacity of the three basidiomycete laccases to decolorize flexographic inks as compared with M. thermophila laccase, a low redox potential laccase. Basidiomycete laccases decolorized inks without mediators at long reaction times, although the presence of natural or synthetic mediators (above all HBT) accelerated the process. On the other hand, M. thermophila laccase was unable to decolorize the inks assayed. The addition of syringyl-type mediators led to medium levels of decolorization except for R48 ink, which was almost completely decolorized.Most decolorization was obtained during the first hours of treatment, when all the basidiomycete laccases were fully active in the presence of mediators. As opposed to other basidiomycete laccases, which become inactive in the presence of HBT after 24 h, the enzyme of P. coccineus was not deactivated by this mediator even after 48 h. A complete loss of M. thermophila laccase activity was observed at short times with acetosyringone and methyl syringate, the only two mediators able to promote ink decolorization with this enzyme. (C) 2012 Elsevier B.V. All rights reserved.