CD163 macrophage and erythrocyte contents in aspirated deep vein thrombus are associated with the time after onset: a pilot study.
CD163 macrophage and erythrocyte contents in aspirated deep vein thrombus are associated with the time after onset: a pilot study.
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DOI：
10.1186/s12959-016-0122-0
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发表时间：
2016
影响因子：
3.1
通讯作者：
Asada Y
中科院分区：
文献类型：
--
作者：
Furukoji E;Gi T;Yamashita A;Moriguchi-Goto S;Kojima M;Sugita C;Sakae T;Sato Y;Hirai T;Asada Y
溶栓治疗在选定的深静脉血栓形成（DVT）患者中是有效的。因此，识别反映血栓年龄的标志物是特别值得关注的。该初步研究旨在确定一种反映人类吸入性DVT发病后时间的标志物。我们对16例抽吸血栓进行了组织学和免疫化学分析。从发病到误吸的时间范围为5至60天（中位数为13天）。石蜡切片用苏木精和伊红以及纤维蛋白、血型糖蛋白A、整联蛋白α2bβ3、巨噬细胞标志物（CD 68、CD 163和CD 206）、CD 34和平滑肌肌动蛋白（SMA）抗体染色。所有血栓均为血型糖蛋白A、纤维蛋白、整合素α2bβ3、CD 68、CD 163和CD 206免疫阳性，并含有粒细胞。几乎所有的血栓都有小的CD 34或SMA免疫阳性区域。CD 68-和CD 163-免疫阳性细胞数与发病后时间呈正相关，而血型糖蛋白A-免疫阳性面积与发病后时间呈负相关。在双重免疫组化中，CD 163阳性细胞主要存在于CD 68免疫阳性巨噬细胞群体中。CD 163阳性巨噬细胞与血型糖蛋白A、CD 34或SMA阳性细胞富集区紧密定位。结果表明，CD 163、巨噬细胞和红细胞含量可作为判断DVT血栓形成时间的指标。此外，CD 163巨噬细胞可能在静脉血栓形成的组织化过程中发挥作用。本文的在线版本（doi：10.1186/s12959-016-0122-0）包含补充材料，可供授权用户使用。
Thrombolytic therapy is effective in selected patients with deep vein thrombosis (DVT). Therefore, identification of a marker that reflects the age of thrombus is of particular concern. This pilot study aimed to identify a marker that reflects the time after onset in human aspirated DVT. We histologically and immunohistochemically analyzed 16 aspirated thrombi. The times from onset to aspiration ranged from 5 to 60 days (median of 13 days). Paraffin sections were stained with hematoxylin and eosin and antibodies for fibrin, glycophorin A, integrin α2bβ3, macrophage markers (CD68, CD163, and CD206), CD34, and smooth muscle actin (SMA). All thrombi were immunopositive for glycophorin A, fibrin, integrin α2bβ3, CD68, CD163, and CD206, and contained granulocytes. Almost all of the thrombi had small foci of CD34- or SMA-immunopositive areas. CD68- and CD163-immunopositive cell numbers were positively correlated with the time after onset, while the glycophorin A-immunopositive area was negatively correlated with the time after onset. In double immunohistochemistry, CD163-positive cells existed predominantly among the CD68-immunopositive macrophage population. CD163-positive macrophages were closely localized with glycophorin A, CD34, or SMA-positive cell-rich areas. These findings indicate that CD163 macrophage and erythrocyte contents could be markers for evaluation of the age of thrombus in DVT. Additionally, CD163 macrophages might play a role in organization of the process of venous thrombus. The online version of this article (doi:10.1186/s12959-016-0122-0) contains supplementary material, which is available to authorized users.