Transgenerational adaptation of Arabidopsis to stress requires DNA methylation and the function of Dicer-like proteins.
Transgenerational adaptation of Arabidopsis to stress requires DNA methylation and the function of Dicer-like proteins.
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DOI：
10.1371/journal.pone.0009514
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发表时间：
2010-03-03
期刊：
影响因子：
3.7
通讯作者：
Kovalchuk I
中科院分区：
文献类型：
--
作者：
Boyko A;Blevins T;Yao Y;Golubov A;Bilichak A;Ilnytskyy Y;Hollunder J;Meins F Jr;Kovalchuk I
哺乳动物和种子植物的表观遗传状态和某些环境反应可以持续到下一代。这些跨代效应具有潜在的适应意义以及医学和农学影响。最近的证据表明，植物的一些非生物和生物胁迫反应是跨代的。例如，烟草植物的病毒感染和拟南芥植物暴露于UVC和鞭毛蛋白可以诱导同源重组频率（HRF）的跨代增加。在这里，我们表明，暴露在拟南芥植物的压力，包括盐，紫外线，冷，热和洪水，导致在一个更高的HRF，增加全球基因组甲基化，并在未经处理的后代更高的耐受性。然而，这种代际效应并没有持续到下一代。用5-氮杂胞苷处理受胁迫植物的后代显示出降低整体基因组甲基化并增强胁迫耐受性。DCL 2和DCL 3编码对小RNA依赖性基因沉默重要的Dicer活性。胁迫诱导的HRF和DNA甲基化在dcl 2和dcl 3缺陷突变体中受损，而在dcl 2突变体中，仅胁迫诱导的胁迫耐受性受损。我们的研究结果与拟南芥中胁迫诱导的跨代反应依赖于DNA甲基化和smRNA沉默途径改变的假设是一致的。
Epigenetic states and certain environmental responses in mammals and seed plants can persist in the next sexual generation. These transgenerational effects have potential adaptative significance as well as medical and agronomic ramifications. Recent evidence suggests that some abiotic and biotic stress responses of plants are transgenerational. For example, viral infection of tobacco plants and exposure of Arabidopsis thaliana plants to UVC and flagellin can induce transgenerational increases in homologous recombination frequency (HRF). Here we show that exposure of Arabidopsis plants to stresses, including salt, UVC, cold, heat and flood, resulted in a higher HRF, increased global genome methylation, and higher tolerance to stress in the untreated progeny. This transgenerational effect did not, however, persist in successive generations. Treatment of the progeny of stressed plants with 5-azacytidine was shown to decrease global genomic methylation and enhance stress tolerance. Dicer-like (DCL) 2 and DCL3 encode Dicer activities important for small RNA-dependent gene silencing. Stress-induced HRF and DNA methylation were impaired in dcl2 and dcl3 deficiency mutants, while in dcl2 mutants, only stress-induced stress tolerance was impaired. Our results are consistent with the hypothesis that stress-induced transgenerational responses in Arabidopsis depend on altered DNA methylation and smRNA silencing pathways.