MUC1, MUC2, MUC4, and MUC5AC expression in salivary gland mucoepidermoid carcinoma - Diagnostic and prognostic implications
MUC1, MUC2, MUC4, and MUC5AC expression in salivary gland mucoepidermoid carcinoma - Diagnostic and prognostic implications
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DOI：
10.1097/01.pas.0000159103.95360.e8
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发表时间：
2005-07-01
影响因子：
5.6
通讯作者：
Fouret, P
中科院分区：
文献类型：
--
作者：
Handra-Luca, A;Lamas, G;Fouret, P
我们确定粘蛋白免疫染色是否可以更好地表征唾液腺粘液表皮样癌（MEC）。我们通过免疫组织化学研究了 63 个 MEC 的 MUC1、MUC2、MUC4 和 MUC5AC。记录表达粘蛋白的细胞类型和标记模式。将结果与显微镜分级、肿瘤相关淋巴浸润、周围唾液腺粘蛋白表达、临床特征和结果进行比较。 MUC1和MUC4标记腺肿瘤细胞的顶膜以及中间、透明和表皮样肿瘤细胞的整个膜。 MUC2和MUC5AC在腺体、粘液和中间肿瘤细胞的细胞质中表达。与 MUC1 相比，MUC4 表达随着肿瘤分级而降低（P < 0.01）。与MUC2不同，MUC5AC在超过50%的高级别肿瘤中表达，其中包括2例未用阿尔新蓝染色的病例。 MUC1 和 MUC5AC 与肿瘤相关淋巴浸润相关（P < 0.05），但与肿瘤相关淋巴滤泡无关。表达粘蛋白的肿瘤比例为：MUC1 为 71%，MUC2 为 21%，MUC4 为 79%，MUC5AC 为 68%。 MUC1 和 MUC5AC 在肿瘤中的表达频率高于周围腺体的表达频率 (P < 0.0001)。 MUC1 表达与较短的无进展生存期相关（P < 0.05）。总之，MEC 中的粘蛋白表达与唾液腺中的不同。中间细胞在其细胞表面表达 MUC1 和 MUC4，在细胞质中表达 MUC2 和 MUC5AC。高级别肿瘤中的 MUC5AC 染色有助于区分高级别 MEC 和鳞状细胞癌。虽然MUC4与肿瘤分化相关，但MUC1表达表明预后较差。
We determined whether immunostaining for mucins could provide a better characterization of salivary gland mucoepidermoid carcinoma (MEC). We investigated 63 MECs by immunohistochemistry for MUC1, MUC2, MUC4, and MUC5AC. Mucin expressing cell types and labeling patterns were recorded. The results were compared with microscopic grade, tumor-associated lymphoid infiltrate, mucin expression in surrounding salivary glands, clinical features, and outcome. MUC1 and MUC4 labeled the apical membrane of glandular tumor cells and the entire membrane of intermediate, clear, and epidermoid tumor cells. MUC2 and MUC5AC were expressed in the cytoplasm of glandular, mucous, and intermediate tumor cells. In contrast to MUC1, MUC4 expression decreased with tumor grade (P < 0.01). Unlike MUC2, MUC5AC was expressed in more than 50% of high-grade tumors, including 2 cases that were not stained with Alcian blue. MUC1 and MUC5AC were associated with tumor-associated lymphoid infiltrates (P < 0.05), but not with tumor-associated lymphoid follicles. The proportions of tumors expressing mucins were 71% for MUC1, 21% for MUC2, 79% for MUC4, and 68% for MUC5AC. MUC1 and MUC5AC were more frequently expressed in tumors than in surrounding glands (P < 0.0001). MUC1 expression correlated with shorter progression-free survival (P < 0.05). In conclusion, mucin expression in MEC differs from that in salivary glands. Intermediate cells express MUC1 and MUC4 all along their cell surface and MUC2 and MUC5AC in their cytoplasm. Staining for MUC5AC in high-grade tumors can be helpful for distinguishing high-grade MEC from squamous cell carcinoma. While MUC4 is related to tumor differentiation, MUC1 expression indicates a worse prognosis.