ENZYMES RELATED TO LIPOGENESIS IN ADIPOSE-TISSUE OF OBESE SUBJECTS
ENZYMES RELATED TO LIPOGENESIS IN ADIPOSE-TISSUE OF OBESE SUBJECTS
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DOI：
10.1016/0026-0495(76)90001-9
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发表时间：
1976-01-01
影响因子：
9.8
通讯作者：
RABUAZZO, AM
中科院分区：
文献类型：
--
作者：
BELFIORE, F;BORZI, V;RABUAZZO, AM
在一组 10 名成年肥胖受试者中，维持正常热量摄入和均衡饮食 15 天，与匹配的正常人相比，脂肪组织中己糖激酶 (EC 2.7.1.1)、6-磷酸果糖激酶 (EC 2.7.1.11) 和 ATP 柠檬酸裂解酶 (EC 4.1.3.8) 的活性显着增加，无论是在蛋白质还是脂肪细胞数量上。科目。 G-6-P脱氢酶(EC 1.1.1.49)、苹果酸脱氢酶(EC 1.1.1.37)和苹果酸脱氢酶(脱羧)(NADP)(EC 1.1.1.40)的活性未改变。由于己糖激酶和6-磷酸果糖激酶均是糖酵解中的速率限制酶，因此它们增强的活性将表明代谢葡萄糖并因此产生α-甘油磷酸的能力增加。 ATP 柠檬酸裂解酶的升高表明脂肪生成增加，因为该酶在脂肪酸合成中发挥调节作用。 G-6-P脱氢酶和苹果酸脱氢酶（脱羧）（NADP）的正常活性为乙酰辅酶A还原为脂肪酸提供NADPH，这表明脂肪生成的变化是中等程度的，因此仅影响最限速的酶ATP柠檬酸裂解酶。这些数据总体上与甘油三酯形成增强的发生一致。观察到的酶变化本质上是适应性的还是遗传性的仍有待澄清。
In a group of 10 adult obese subjects, maintained for 15 days on a normal caloric intake and balanced diet, the activity of hexokinase (EC 2.7.1.1), 6-phosphofructokinase (EC 2.7.1.11) and ATP citrate-lyase (EC 4.1.3.8) in the adipose tissue was significantly increased, both on a protein and on a fat cell number basis, compared to matched normal subjects. The activity of G-6-P dehydrogenase (EC 1.1.1.49), malate dehydrogenase (EC 1.1.1.37) and malate dehydrogenase (decarboxylating) (NADP) (EC 1.1.1.40) was unchanged. Since both hexokinase and 6-phosphofructokinase are rate-liniting in glycolysis, their enhanced activity would indicate the occurrence of an increased capacity to metabolize glucose and therefore to generate .alpha.-glycerophosphate. The elevation of ATP citrate-lyase would suggest increased lipogenesis, owing to the regulatory role that this enzyme plays in fatty acid synthesis. The normal activity of G-6-P dehydrogenase and malate dehydrogenase (decarboxylating) (NADP), which supply NADPH for the reduction of acetyl-CoA to fatty acids, would suggest that the change in lipogenesis is of moderate degree, thereby affecting only the most rate-limiting enzyme, ATP citrate-lyase. These data, on the whole, are consistent with the occurrence of enhanced triglyceride formation. Whether the enzyme changes observed are adaptive or genetic in nature remains to be clarified.