Morpholino-mediated gene knockdown in mammalian organ culture.
Morpholino-mediated gene knockdown in mammalian organ culture.
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DOI：
10.1007/978-1-61779-851-1_27
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Tufro, Alda
中科院分区：
文献类型：
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作者：
Tufro, Alda
We examined the role of semaphorin3a in ureteric bud (UB) branching morphogenesis using mouse metanephric organ culture [Tufro et al. (Mech Dev 125:558–568, 2008)]. In vitro UB injection of Sema3a antisense morpholino resulted in increased branching morphogenesis. Cellular and tissue uptake of oligonucleotides was facilitated by a peptide-mediated method. Our findings were validated by in vitro translation and in Sema3a null mice. This chapter describes a method to perfuse the UB lumen with fluorescein-labeled oligonucleotides bound to a peptide carrier.
