Comparison of improved precipitation methods for quantification of high-density lipoprotein cholesterol.
Comparison of improved precipitation methods for quantification of high-density lipoprotein cholesterol.
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DOI：
10.1093/clinchem/31.2.217
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发表时间：
1985-02
影响因子：
9.3
通讯作者：
Russell Warnicl;Thuy Nguyen;Alegrla A. Albers
中科院分区：
文献类型：
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作者：
Russell Warnicl;Thuy Nguyen;Alegrla A. Albers
我们比较了标准的脂质研究临床肝素锰（46 mmol/L）的方法和五个改进的沉淀方法定量高密度脂蛋白（HDL）胆固醇。这些方法中的三种-葡聚糖硫酸盐-Mg 2+程序，报告为选定的方法，改良的肝素-Mn 2+（92 mmol/L）方法和改良的磷钨酸盐-Mg 2+程序-都得到了类似的结果。其他三种方法-标准肝素-Mn 2+（46 mmol/L）方法和两种聚乙二醇方法（75 g/L或pH 10试剂，最终浓度为100 g/L）-对HDL胆固醇的测定值略高。加入NaCl或葡萄糖的标本没有显着改变蛋白质沉淀。在高胆红素血症标本的沉降效果方面，方法按以下顺序排列：聚乙二醇（pH 10，100 g/L）大于硫酸葡聚糖-Mg 2+大于肝素-Mn 2+（92 mmol/L）=聚乙二醇（75 g/L）大于磷钨酸盐-Mg 2+大于肝素-Mn 2+（46 mmol/L）。
We compared the standard Lipid Research Clinics heparin-Mn2+ (46 mmol/L) method and five improved precipitation methods for quantification of high-density lipoprotein (HDL) cholesterol. Three of these methods--a dextran sulfate-Mg2+ procedure, reported as a Selected Method, a modified heparin-Mn2+ (92 mmol/L) method, and a modified phosphotungstate-Mg2+ procedure--all gave similar results. Three other methods--the standard heparin-Mn2+ (46 mmol/L) method and two polyethylene glycol methods (75 g/L or pH 10 reagent at 100 g/L final concentrations)--gave slightly higher values for HDL cholesterol. Addition of NaCl or glucose to specimens did not significantly change protein precipitation. In terms of sedimentation effectiveness with hypertriglyceridemic specimens, the methods were ranked in the following order: polyethylene glycol (pH 10, 100 g/L) greater than dextran sulfate-Mg2+ greater than heparin-Mn2+ (92 mmol/L) = polyethylene glycol (75 g/L) greater than phosphotungstate-Mg2+ greater than heparin-Mn2+ (46 mmol/L).