OBSERVATIONS ON THE SOCIAL BEHAVIOUR OF CELLS IN TISSUE CULTURE .2. MONOLAYERING OF FIBROBLASTS
OBSERVATIONS ON THE SOCIAL BEHAVIOUR OF CELLS IN TISSUE CULTURE .2. MONOLAYERING OF FIBROBLASTS
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DOI：
10.1016/0014-4827(54)90176-7
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发表时间：
1954-01-01
影响因子：
3.7
通讯作者：
HEAYSMAN, JEM
中科院分区：
文献类型：
--
作者：
ABERCROMBIE, M;HEAYSMAN, JEM
1.1.观察了两个鸡胚胎心脏外植体之间的细胞行为，在液体培养中相隔0.5至1 mm。在这种情况下，当两个外植体的细胞入侵时，给定区域的细胞数量从零稳步上升，但在相反的外植体相遇后不久，细胞数量几乎停止上升。当细胞接近时，在突起实际接合之前，没有检测到种群增长率的下降。外植体连接后单个细胞的运动速度在外植体之间比在外植体两侧要慢得多。外植体之间区域中细胞的运动方向主要是从各自的外植体向外移动，直到两个长出的细胞结合在一起，然后在所有方向上变得几乎随机不同。结果表明，在相对的细胞片之间建立连接后不久，向两个外植体之间的区域的突起几乎停止。在这种连接之后，连接两个外植体的径向条带中的细胞数量被两个径向条带的总和所超过，每个外植体的边上一条。因此，在两个外显者之间的文化外延基本上停止后，文化外延继续横向发展。接合后，两个外植体之间的细胞趋于“单层”。如果细胞是随机分布的，一个核与另一个核重叠的情况比预期的要少得多。结论是，成纤维细胞避免在彼此的表面上移动。这种对运动的“接触抑制”可以解释为什么成纤维细胞通常主要是从外植体径向迁移，而整个培养过程无论最初的形式如何，都会迅速变得环状。
1.1. Observations have been made on cell behaviour in the area between two embryonic chick heart explants, placed 0.5 to 1 mm apart in liquid medium tissue culture.2.2. The cell population in a given area in this situation rises steadily from zero as cells from both explants invade it, but then almost ceases to rise soon after the opposing outgrowths have met. There is no detectable diminution of rate of rise of population while the cells are approaching, before the outgrowths actually join.3.3. The velocity of individual cell movement after junction of the outgrowths is much slower between the explants than at either side of the explants.4.4. The direction of movements of cells in the region between the explants is predominantly outward from their respective explants until the two out-growths of cells join, when it becomes little more than randomly different from equal in all directions.5.5. It is concluded that outgrowth into the area between the two explants almost ceases soon after junction has been established between the opposing sheets of cells. After this junction, the number of cells in a radial strip joining the two explants becomes exceeded by the sum of two radial strips one at the side of each explant. Culture outgrowth therefore continues laterally after it has substantially ceased between the two explants.6.6. After junction the cells between the two explants tend to remain as a “monolayer”. The number of instances where one nucleus overlaps another is much fewer than is to be expected if the cells were randomly distributed.7.7. It is concluded that fibroblasts avoid moving over each other's surfaces. Such “contact-inhibition” of movement can explain why it is that fibroblasts normally migrate predominantly radially from an explant and that the whole culture tends rapidly to become circular in plan whatever its initial form.