The expression of platelet-derived endothelial cell growth factor/thymidine phosphorylation associates with angiogenesis in epithelial ovarian cancer
The expression of platelet-derived endothelial cell growth factor/thymidine phosphorylation associates with angiogenesis in epithelial ovarian cancer
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DOI：
10.1007/bf02488995
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发表时间：
1997-12-01
影响因子：
3.3
通讯作者：
Kudo, Takafumi
中科院分区：
文献类型：
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作者：
Nakanishi, Yoshie;Kodama, Junichi;Kudo, Takafumi
背景：本研究的目的是阐明血管生成因子 PD-ECGF（血小板源性内皮细胞生长因子/胸苷磷酸化酶）与上皮性卵巢癌中的临床病理因素（在本例中为肿瘤血管生成）之间的关系。方法：取60例上皮性卵巢癌患者手术时原发灶的肿瘤标本。根据世界卫生组织的分类，将组织学细胞类型分配给肿瘤：26个被分类为浆液性腺癌，15个被分类为子宫内膜样腺癌，9个被分类为粘液性腺癌，9个被分类为透明细胞癌，1个被分类为未分化癌。手术分期基于国际妇产科联合会（FIGO）分期系统：16例为1期，6例为II期，34例为III期，4例为IV期。通过免疫组织化学染色评估PD-ECGF的表达。通过对新生血管最多的区域中因子 VIII 相关抗原的免疫染色来评估微血管密度。结果：基质细胞比癌细胞染色更强烈（80% vs. 33%）。在晚期癌症病例中，基质细胞中 PD-ECGF 的免疫阳性率更高。黏液性腺癌中PD-ECGF的表达显着高于浆液性腺癌，而透明细胞癌中PD-ECGF的表达显着降低。 PD-ECGF阳性基质细胞明显的病例的微血管密度显着高于PD-ECGF阳性基质细胞缺失/极少的病例（P < 0.05）。结论：PD-ECGF的表达可能在促进上皮性卵巢癌血管生成中发挥重要作用。
Background: The object of this study is to clarify the association of an angiogenic factor, PD-ECGF (platelet-derived endothelial cell growth factor/thymidine phosphorylase), with clinicopathologic factors, in this case tumor angiogenesis, in epithelial ovarian cancers. Methods: Tumor specimens were obtained at the time of surgery from the primary lesion in 60 patients with epithelial ovarian cancer. Histologic cell types were assigned to tumors according to the World Health Organization classification: 26 were classified as serous adenocarcinoma, 15 as endometrioid adenocarcinoma, 9 as mucinous adenocarcinoma, 9 as clear cell carcinoma, and 1 as undifferentiated carcinoma. Surgical staging was based on the International Federation of Gynecology and Obstetrics (FIGO) staging system: 16 were stage 1, 6 were stage II, 34 were stage III, and 4 were stage IV. Expression of PD-ECGF was evaluated by immunohistochemical staining. Microvessel density was assessed by immunostaining for factor VIII-related antigen in the most neovascularized area. Results: Stroma cells stained more strongly than cancer cells (80% vs. 33%). The immunopositivity of PD-ECGF in stroma cells was higher in cases of advanced cancer. Expression of PD-ECGF in mucinous adenocarcinomas was significantly higher than that in serous adenocarcinomas, while PD-ECGF expression in clear cell carcinomas was significantly lower. The microvessel density in the cases with marked PD-ECGF-positive stroma cells was significantly higher than that in the cases with absent/ minimal PD-ECGF-positive stroma cells (P < 0.05). Conclusion: The expression of PD-ECGF may play a crucial role in the promotion of angiogenesis in epithelial ovarian cancers.