Curcumin-mediated photodynamic inactivation of Candida albicans in a murine model of oral candidiasis
Curcumin-mediated photodynamic inactivation of Candida albicans in a murine model of oral candidiasis
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DOI：
10.3109/13693786.2012.714081
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发表时间：
2013-04-01
期刊：
影响因子：
2.9
通讯作者：
Pavarina, Ana Claudia
中科院分区：
文献类型：
--
作者：
Dovigo, Livia Nordi;Carmello, Juliana Cabrini;Pavarina, Ana Claudia
姜黄素介导的光动力疗法（PDT）的体外研究令人鼓舞，但缺乏可靠的体内疗效证据。本研究描述了白色念珠菌在口腔念珠菌病小鼠模型中的光失活，使用姜黄素作为光敏剂。将40只免疫抑制小鼠口服接种白色念珠菌，5天后给予姜黄素（20、40和80 μ M）和LED灯照射。还研究了姜黄素或光单独使用的效果。阳性对照动物不接受任何治疗，阴性对照动物不接种白色念珠菌。酵母存活细胞数采用方差分析和Tukey事后检验（α = 0.05）进行分析。组织学评估的存在酵母和炎症反应也进行了。所有暴露于LED光下的姜黄素都导致PDT后白色假丝酵母菌活力显著降低，但使用80 μ M姜黄素与光相结合能够诱导菌落计数减少最高的对数10（4对数）。结果表明，姜黄素介导的PDT可有效灭活白色念珠菌，且不损伤小鼠宿主组织。
In vitro investigations of curcumin-mediated photodynamic therapy (PDT) are encouraging, but there is a lack of reliable in vivo evidence of its efficacy. This study describes the photoinactivation of Candida albicans in a murine model of oral candidiasis, using curcumin as a photosensitizer. Forty immunosuppressed mice were orally inoculated with C. albicans and after five days, they received topical curcumin (20, 40 and 80 mu M) and illumination with LED light. The use of curcumin or light alone were also investigated. Positive control animals did not receive any treatment and negative control animals were not inoculated with C. albicans. The number of surviving yeast cells was determined and analyzed by ANOVA and Tukey's post-hoc test (alpha = 0.05). Histological evaluation of the presence of yeast and inflammatory reaction was also conducted. All exposures to curcumin with LED light caused a significant reduction in C. albicans viability after PDT, but the use of 80 mu M curcumin associated with light was able to induce the highest log 10 reduction in colony counts (4 logs). It was concluded that curcumin-mediated PDT proved to be effective for in vivo inactivation of C. albicans without harming the host tissue of mice.