Response of the lysosomal system of the corneal epithelium to tyrosine-induced cell injury.
Response of the lysosomal system of the corneal epithelium to tyrosine-induced cell injury.
复制标题
角膜上皮溶酶体系统对酪氨酸诱导的细胞损伤的反应。
DOI：
10.1177/25.12.925342
复制
发表时间：
1977
期刊：
影响因子：
--
通讯作者：
E. Squires
中科院分区：
文献类型：
--
作者：
M. Beard;R. Burns;L. Rich;E. Squires
喂食过量酪氨酸的大鼠发生角膜上皮疾病，与人类酪氨酸转氨酶缺乏症（酪氨酸病）相似。在大鼠中，中央上皮内出现局灶性病变，并含有破坏细胞的晶体（推测为酪氨酸）。我们研究了这些病变和本地化的酸性磷酸酶和芳基硫酸酯酶在电子显微镜水平。在开始饮食后60小时内，病变内的细胞显示溶酶体酶活性增加。这种活性位于一些晶体鬼和许多溶酶体内，包括自噬空泡和多泡体。喂食84小时后，整个中央角膜上皮被破坏，多形核白细胞浸润该区域。晶体被多形核白细胞吞噬或在多形核白细胞内形成。我们假设晶体在上皮细胞内形成，首先破坏它们的溶酶体，然后破坏细胞，导致溶酶体酶的外化。这种细胞外溶酶体酶的释放可能是引起急性炎症反应的原因。
Rats fed excess tyrosine develop corneal epithelial disease which parallels that found in humans with tyrosine aminotransferase deficiency (tyrosinosis). In the rat, focal lesions develop within the central epithelium and contain crystals (presumably tyrosine) that disrupt cells. We have studied these lesions and localized acid phosphatase and aryl sulfatase at the electron microscope level. Within 60 hr after initiation of diet, cells within the lesions showed an increase in lysosomal enzyme activity. This activity was localized inside some of the crystal ghosts and in numerous lysosomes, including autophagic vacuoles and multivesicular bodies. After 84 hr on diet the entire central corneal epithelium was disrupted and polymorphonuclear leukocytes had infiltrated the area. Crystals were phagocytosed by or developed within polymorphonuclear leukocytes. We hypothesize that crystals form within epithelial cells, disrupt first their lysosomes, and then cells, leading to externalization of lysosomal enzymes. This extracellular lysosomal enzyme release may be responsible for the acute inflammatory response that ensues.