SELECTIVE DEPOSITION OF IMMUNOGLOBULIN-A1 IN IMMUNOGLOBULIN-A NEPHROPATHY, ANAPHYLACTOID PURPURA NEPHRITIS, AND SYSTEMIC LUPUS-ERYTHEMATOSUS
SELECTIVE DEPOSITION OF IMMUNOGLOBULIN-A1 IN IMMUNOGLOBULIN-A NEPHROPATHY, ANAPHYLACTOID PURPURA NEPHRITIS, AND SYSTEMIC LUPUS-ERYTHEMATOSUS
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DOI：
10.1172/jci109998
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发表时间：
1980-01-01
影响因子：
15.9
通讯作者：
MICHAEL, AF
中科院分区：
文献类型：
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作者：
CONLEY, ME;COOPER, MD;MICHAEL, AF
为了表征伊加肾病、过敏性紫癜性肾炎和系统性红斑狼疮患者肾小球中发现的伊加沉积物，用单克隆抗IgA亚类试剂、抗L链试剂和抗J链对这些疾病患者的肾活检进行免疫荧光染色。系膜和外周毛细血管对IgA 1呈明亮染色，对IgA 2呈阴性。IgA 1，并在较小程度上，IgA 2包含在管状管型。两个都是。卡帕。和. da。在所有沉积物中均发现了L链。J链染色强度与IgM染色强度相关，而与伊加染色强度无关。活检组织伊加染色明亮，但IgM阴性，J链阴性。显然，这些疾病中的肾小球伊加沉积物主要由IgA 1单体组成。
To characterize the IgA deposits found in glomeruli of patients with IgA nephropathy, anaphylactoid purpura nephritis, and systemic lupus erythematosus, renal biopsies from patients with these disorders were stained by immunofluorescence with monoclonal anti-IgA subclass reagents, anti-L chain reagents and anti-J chain. The mesangium and peripheral capillary were brightly stained for IgA1 and were negative for IgA2. IgA1 and, to a lesser extent, IgA2 were contained in tubular casts. Both .kappa. and .lambda. L chains were found in all deposits. The intensity of J chain staining correlated with the intensity of IgM and not IgA staining. Biopsies brightly stained for IgA but negative for IgM were negative for J chain. Apparently glomerular IgA deposits in these disorders consist predominantly of IgA1 monomers.