Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection
Inhibition of Trypanosoma cruzi proline racemase affects host-parasite interactions and the outcome of in vitro infection
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DOI：
10.1590/s0074-02762009000800001
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发表时间：
2009-12-01
期刊：
影响因子：
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通讯作者：
Soeiro, Maria de Nazaré Correia
中科院分区：
文献类型：
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作者：
Coutinho, Leticia;Ferreira, Marcelo Alves;Soeiro, Maria de Nazaré Correia
脯氨酸消旋酶是克氏锥虫的重要酶，并且已被证明是B细胞的有效有丝分裂原，从而有助于寄生虫的免疫逃避和在人类宿主中的持久性。过表达编码脯氨酸消旋酶的TcPRAC基因的重组外鞭毛体寄生虫呈现出增强的分化成亚循环感染形式并随后在体外穿透宿主细胞的能力。在这里，我们证明，无论是反T。Cruzi脯氨酸消旋酶抗体和特异性脯氨酸消旋酶抑制剂吡咯-2-羧酸显著影响寄生虫体外感染Vero细胞。这种抑制剂也阻碍T. cruzi细胞内分化
Proline racemase is an important enzyme of Trypanosoma cruzi and has been shown to be an effective mitogen for B cells, thus contributing to the parasite's immune evasion and persistence in the human host. Recombinant epimastigote parasites overexpressing TcPRAC genes coding for proline racemase present an augmented ability to differentiate into metacyclic infective forms and subsequently penetrate host-cells in vitro. Here we demonstrate that both anti T. cruzi proline racemase antibodies and the specific proline racemase inhibitor pyrrole-2-carboxylic acid significantly affect parasite infection of Vero cells in vitro. This inhibitor also hampers T. cruzi intracellular differentiation.