Assessing genetic diversity and similarity of 435 KPC-carrying plasmids
Assessing genetic diversity and similarity of 435 KPC-carrying plasmids
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DOI：
10.1038/s41598-019-47758-5
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发表时间：
2019-08-02
影响因子：
4.6
通讯作者：
Makarewicz, Oliwia
中科院分区：
文献类型：
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作者：
Brandt, Christian;Viehweger, Adrian;Makarewicz, Oliwia
多重耐药革兰氏阴性（MRGN）细菌的全球传播和多样化对医疗保健构成了重大挑战。特别是，碳青霉烯类耐药肺炎克雷伯菌菌株经常在感染和医院范围内的爆发中被发现。最常见的潜在耐药基因（bla（KPC））在过去十年中一直在卫生保健环境中传播。bla（KPC）似乎已经迅速多样化，并且已经在各种物种和不同的质粒类型中发现。为了审查这种多样化的进展和动态，在这项工作中分析了NCBI数据库中所有目前可用的KPC质粒。将质粒分为257个不同的代表性KPC质粒，其中79.4%可以明确地归属于不相容性（Inc）组。在几乎一半的代表性质粒中，KPC基因位于Tn 4401变体上，强调了这种转座子类型对于将KPC基因传递到其他质粒的重要性。转座子似乎也负责发生改变或罕见的融合质粒类型，可能是由于不完全转座。此外，许多KPC质粒含有编码促进重组过程和诱变的蛋白质的基因;从而加速KPC基因和其他共定位抗性基因的多样化。
The global spread and diversification of multidrug-resistant Gram-negative (MRGN) bacteria poses major challenges to healthcare. In particular, carbapenem-resistant Klebsiella pneumoniae strains have been frequently identified in infections and hospital-wide outbreaks. The most frequently underlying resistance gene (bla(KPC)) has been spreading over the last decade in the health care setting. bla(KPC) seems to have rapidly diversified and has been found in various species and on different plasmid types. To review the progress and dynamics of this diversification, all currently available KPC plasmids in the NCBI database were analysed in this work. Plasmids were grouped into 257 different representative KPC plasmids, of which 79.4% could be clearly assigned to incompatibility (Inc) group or groups. In almost half of all representative plasmids, the KPC gene is located on Tn4401 variants, emphasizing the importance of this transposon type for the transmission of KPC genes to other plasmids. The transposons also seem to be responsible for the occurrence of altered or uncommon fused plasmid types probably due to incomplete transposition. Moreover, many KPC plasmids contain genes that encode proteins promoting recombinant processes and mutagenesis; in consequence accelerating the diversification of KPC genes and other colocalized resistance genes.