Stimulation of T-helper cell gamma interferon and immunoglobulin G responses specific for Babesia bovis rhoptry-associated protein 1 (RAP-1) or a RAP-1 protein lacking the carboxy-terminal repeat region is insufficient to provide protective immunity again
Stimulation of T-helper cell gamma interferon and immunoglobulin G responses specific for Babesia bovis rhoptry-associated protein 1 (RAP-1) or a RAP-1 protein lacking the carboxy-terminal repeat region is insufficient to provide protective immunity again
复制标题
对牛巴贝虫棒状体相关蛋白 1 (RAP-1) 或缺乏羧基末端重复区域的 RAP-1 蛋白特异性的 T 辅助细胞 γ 干扰素和免疫球蛋白 G 反应的刺激不足以再次提供保护性免疫
DOI：
10.1128/iai.71.9.5021-5032.2003
复制
发表时间：
2003
影响因子：
3.1
通讯作者：
Brown,WendyC
中科院分区：
文献类型：
--
作者：
Norimine,Junzo;Mosqueda,Juan;Suarez,Carlos;Palmer,GuyH;McElwain,TerryF;Mbassa,Gabriel;Brown,WendyC
Rhoptry-associated protein 1 (RAP-1) is a targeted vaccine antigen forBabesia bovisandBabesia bigeminainfections of cattle. The 60-kDaB. bovisRAP-1 is recognized by antibodies and T lymphocytes from cattle that recovered from infection and were immune to subsequent challenge. Immunization with native or recombinant protein was reported to reduce parasitemias in challenged animals. We recently reported that the NT domain ofB. bovisRAP-1 contained immunodominant T-cell epitopes, whereas the repeat-rich CT domain was less immunostimulatory for T lymphocytes from cattle immune toB. bovis.The present study was therefore designed to test the hypothesis that the NT region of RAP-1, used as a vaccine with interleukin-12 and RIBI (catalog no. R-730; RIBI Immunochem Research, Inc., Hamilton, Mont. [now Corixa, Seattle, Wash.]) adjuvant to induce a type 1 response, would prime calves for antibody and T-helper cell responses comparable to or greater than those induced by full-length RAP-1 containing the C-terminal repeats. Furthermore, a type 1 immune response to RAP-1 was hypothesized to induce protection against challenge. Following four inoculations of either recombinant full-length RAP-1 or RAP-1 NT protein, RAP-1-specific immunoglobulin G (IgG) titers, T-lymphocyte proliferation, and gamma interferon production were similar. Similar numbers of NT region peptides were recognized. However, in spite of the presence of strong RAP-1-specific IgG and CD4+-T-lymphocyte responses that were recalled upon challenge, neither antigen stimulated a protective immune response. We conclude that successful priming of calves with recombinant RAP-1 and adjuvants that elicit strong Th1 cell and IgG responses is insufficient to protect calves against virulentB. bovischallenge.
