The effects of lycopene on the proliferation of human breast cells and BRCA1 and BRCA2 gene expression
The effects of lycopene on the proliferation of human breast cells and BRCA1 and BRCA2 gene expression
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DOI：
10.1016/j.ejca.2004.03.028
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发表时间：
2004-07-01
影响因子：
8.4
通讯作者：
Bernard-Gallon, DJ
中科院分区：
文献类型：
--
作者：
Chalabi, N;Le Corre, L;Bernard-Gallon, DJ
本研究的目的是证明番茄红素，主要的番茄类胡萝卜素，对BRCA 1和BRCA 2基因在三个乳腺肿瘤细胞系，MCF-7，HBL-100，MDA-MB-231和纤维囊性乳腺细胞系MCF-10a的表达的影响。流式细胞仪分析显示，用10 μ M番茄红素处理细胞48 h后，细胞周期停滞在G（1）/S期。使用Taqman（R）方法通过定量逆转录-聚合酶链反应研究mRNA表达。我们观察到BRCA 1和BRCA 2 mRNA在雌激素受体（ER）阳性细胞系（MCF-7和HBL-100）中增加，而在ER阴性细胞系中减少（MDA-MB-231）或无变化（MCF-10a）。BRCA 1和BRCA 2蛋白通过灌注亲和层析进行定量。未观察到其表达的变化。这些关于番茄红素对乳腺癌中BRCA 1和BRCA 2抑癌基因表达影响的初步结果可能反映了雌激素和维甲酸受体（RAR）途径之间的相互作用。(C)2004 Elsevier Ltd.保留所有权利。
The purpose of this study was to demonstrate the effects of lycopene, the major tomato carotenoid, on the expression of the BRCA1 and BRCA2 genes in three breast tumour cell lines, MCF-7, HBL-100, MDA-MB-231 and the fibrocystic breast cell line MCF-10a. Flow cytometry analysis showed a G(1)/S phase cell cycle-arrest after treatment of the cells with 10 muM lycopene for 48 h. mRNA expression was studied by quantitative reverse transcription-polymerase chain reaction using the Taqman(R) method. We observed an increase of BRCA1 and BRCA2 mRNA in the oestrogen receptor (ER)-positive cell lines (MCF-7 and HBL-100), and a decrease (MDA-MB-231) or no change (MCF-10a) in the ER-negative cell lines. BRCA1 and BRCA2 proteins were quantified by perfusion affinity chromatography. No variation in their expression was observed. These preliminary results on the effects of lycopene on the expression of BRCA1 and BRCA2 oncosuppressor genes in breast cancer may reflect cross-talk between the oestrogen and retinoic acid receptor (RAR) pathways. (C) 2004 Elsevier Ltd. All rights reserved.