Mapping quantitative trait loci using four-way crosses
Mapping quantitative trait loci using four-way crosses
复制标题
DOI：
10.1017/s0016672300034066
复制
发表时间：
1996-10-01
期刊：
影响因子：
--
通讯作者：
Xu, SZ
中科院分区：
文献类型：
--
作者：
Xu, SZ
在植物物种中，典型的基因定位策略使用从两个近交系之间的杂交开始的群体。然而，可以使用包括两个以上父母的计划。本文介绍了一种新的方法，它使用一个四元杂交群体来自四个自交系。利用四元杂交设计进行QTL定位，可以在一个试验中同时检测四个品系的QTL分离。因此，这是一个更经济的战略比一个使用线之间的交叉只有两个线。新策略还增加了检测QTL的概率，如果他们分离在一个线交叉，但不是在其他。多元线性回归分析用于QTL检测。证明了回归分析的期望残差方差不同于纯环境方差。提出了校正偏差的方法，并通过计算机模拟进行了验证。
In plant species, typical gene mapping strategies use populations initiated from crosses between two inbred lines. However, schemes including more than two parents could be used. In this paper, a new approach is introduced which uses a four-way cross population derived from four inbred lines. The four-way cross design for mapping quantitative trait loci (QTLs) provides tests for QTL segregation in four lines simultaneously in one experiment. Therefore, it is a more economical strategy than one using line crosses between only two lines. The new strategy also increases the probability of detecting QTLs if they segregate in one line cross but not in the other. A multiple linear regression analysis is used for QTL detection. It is proven that the expected residual variance from the regression analysis differs from the pure environmental variance. Correction for the bias is proposed and verified by computer simulations.