A comprehensive, genome-wide analysis of autophagy-related genes identified in tobacco suggests a central role of autophagy in plant response to various environmental cues.
A comprehensive, genome-wide analysis of autophagy-related genes identified in tobacco suggests a central role of autophagy in plant response to various environmental cues.
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对烟草中发现的自噬相关基因进行的全面、全基因组分析表明，自噬在植物对各种环境因素的反应中发挥着核心作用
DOI：
10.1093/dnares/dsv012
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发表时间：
2015-08
期刊：
影响因子：
--
通讯作者：
Sun MX
中科院分区：
文献类型：
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作者：
Zhou XM;Zhao P;Wang W;Zou J;Cheng TH;Peng XB;Sun MX
自噬是一种在动物和植物中进化上保守的机制，参与了植物的多种重要发育过程。烟草被认为是一种理想的模式植物，已被广泛用于研究自噬在植物发育过程中的作用和对各种胁迫的响应。然而，到目前为止，只有少数自噬相关基因（ATGs）已被确定在烟草。在这里，我们通过全基因组调查确定了烟草中属于16个不同组的30个ATG。全面的表达谱分析揭示了这些ATG的广泛表达模式，在正常生长条件下可以在所有测试的组织中检测到。我们的系列测试进一步揭示了大多数ATGs对不同的胁迫（包括营养饥饿、植物激素、重金属和其他非生物胁迫）敏感并响应，表明自噬可能作为效应器在植物对各种环境信号的响应中起核心作用。这项工作提供了烟草中所有ATG的详细调查，也表明了自噬在正常植物生长和植物对环境胁迫的反应中的多种功能。
Autophagy is an evolutionarily conserved mechanism in both animals and plants, which has been shown to be involved in various essential developmental processes in plants. Nicotiana tabacum is considered to be an ideal model plant and has been widely used for the study of the roles of autophagy in the processes of plant development and in the response to various stresses. However, only a few autophagy-related genes (ATGs) have been identified in tobacco up to now. Here, we identified 30 ATGs belonging to 16 different groups in tobacco through a genome-wide survey. Comprehensive expression profile analysis reveals an abroad expression pattern of these ATGs, which could be detected in all tissues tested under normal growth conditions. Our series tests further reveal that majority of ATGs are sensitive and responsive to different stresses including nutrient starvation, plant hormones, heavy metal and other abiotic stresses, suggesting a central role of autophagy, likely as an effector, in plant response to various environmental cues. This work offers a detailed survey of all ATGs in tobacco and also suggests manifold functions of autophagy in both normal plant growth and plant response to environmental stresses.