Processes regulating cervical ripening differ from cervical dilation and postpartum repair: insights from gene expression studies.
Processes regulating cervical ripening differ from cervical dilation and postpartum repair: insights from gene expression studies.
复制标题
DOI：
10.1177/1933719107309587
复制
发表时间：
2007-12-01
期刊：
影响因子：
--
通讯作者：
Mahendroo, Mala
中科院分区：
文献类型：
--
作者：
Timmons, Brenda C;Mahendroo, Mala
需要更好地了解在妊娠、分娩和产后期间调节宫颈重塑的过程，以了解早产和产后分娩的原因，其中宫颈功能异常是罪魁祸首。在目前的研究中，在小鼠模型中鉴定了与宫颈扩张和/或产后修复相比，宫颈成熟特有的基因表达模式。从妊娠第15天到第18天晚些时候差异调节的基因揭示了宫颈成熟的重要过程。从出生后18天晚些时候到2小时差异调节的基因揭示了在宫颈扩张或产后恢复期可能很重要的过程。基于表达模式，宫颈成熟需要胶原蛋白组装基因的下调;破坏基质的糖胺聚糖（如透明质酸）的合成增加;孕酮代谢增加;以及上皮屏障特性的变化。扩张和产后恢复的后期阶段与成熟胶原蛋白的增加组装、促进致密结缔组织的基质蛋白的合成、炎症反应的激活、前列腺素合成以及上皮屏障性质和分化的进一步变化相关。宫颈成熟所需的过程/基因表达与宫颈重塑后期的重要过程/基因表达不同，并突出了组织收集时机对了解宫颈成熟分子机制的重要性。
A greater understanding of the processes that regulate cervical remodeling during pregnancy, parturition, and the postpartum period is required to understand causes of preterm and posterm birth in which abnormal cervical function is the primary culprit. In the current study, gene expression patterns unique to cervical ripening as compared with cervical dilation and/or postpartum repair are identified in a mouse model. Genes differentially regulated from gestation day 15 to late day 18 reveal processes important for cervical ripening. Genes differentially regulated from late day 18 to 2 hours after birth reveal processes that could be important during cervical dilation or the postpartum recovery period. Based on expression patterns, cervical ripening requires a downregulation of collagen assembly genes; increased synthesis of glycosaminoglycans that disrupt the matrix, such as hyaluronan; increased metabolism of progesterone; and changes in epithelial barrier properties. The latter phases of dilation and postpartum recovery are associated with increased assembly of mature collagen, synthesis of matrix proteins that promote a dense connective tissue, activation of inflammatory responses, prostaglandin synthesis, and further changes in epithelial barrier properties and differentiation. Processes/gene expression required for cervical ripening are distinct from those important in latter phases of cervical remodeling and highlight the importance of timing of tissue collection for understanding the molecular mechanisms of cervical ripening.