A highly selective near-infrared fluorescent probe for carboxylesterase 2 and its bioimaging applications in living cells and animals
A highly selective near-infrared fluorescent probe for carboxylesterase 2 and its bioimaging applications in living cells and animals
复制标题
一种高选择性的羧酸酯酶2近红外荧光探针及其在活细胞和动物中的生物成像应用
DOI：
10.1016/j.bios.2016.04.075
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发表时间：
2016-09-15
影响因子：
12.6
通讯作者：
Yang, Ling
中科院分区：
文献类型：
--
作者：
Jin, Qiang;Feng, Lei;Yang, Ling
设计、合成了一种高选择性、高灵敏度检测羧酸酯酶2（CE 2）的近红外荧光探针（DDAB），并对其进行了系统的体内外研究。在加入CE2后，DDAB的酯键可以快速断裂，然后释放近红外（NIR）荧光团DDAO，其在生理溶液中带来显著的黄至蓝颜色变化和强的NIR荧光发射。新开发的探针具有良好的特性，包括良好的特异性，灵敏度和高成像分辨率。DDAB还可用于测定复杂生物样品中CE 2的真实的活性，以及以组织制备物为酶源筛选CE 2抑制剂。该探针还首次成功地用于检测活细胞和体内的内源性CE2，结果表明这种检测是高度可靠的。DDAB的这些突出特点使其在进一步研究CE2相关的生物过程和探索CE2在生命系统中的生理功能方面具有重要的应用前景。(C)2016爱思唯尔B.V.保留所有权利。
A near-infrared fluorescent probe (DDAB) for highly selective and sensitive detection of carboxylesterase 2 (CE2) has been designed, synthesized, and systematically studied both in vitro and in vivo. Upon addition of CE2, the ester bond of DDAB could be rapidly cleaved and then release a near-infrared (NIR) fluorophore DDAO, which brings a remarkable yellow-to-blue color change and strong NIR fluorescence emission in physiological solutions. The newly developed probe exhibits excellent properties including good specificity, ultrahigh sensitivity and high imaging resolution. Moreover, DDAB has been applied to measure the real activities of CE2 in complex biological samples, as well as to screen CE2 inhibitors by using tissue preparations as the enzymes sources. The probe has also been successfully used to detect endogenous CE2 in living cells and in vivo for the first time, and the results demonstrate that such detection is highly reliable. All these prominent features of DDAB make it holds great promise for further investigation on CE2-associated biological process and for exploring the physiological functions of CE2 in living systems. (C) 2016 Elsevier B.V. All rights reserved.