Silver ion bioreduction in nanoparticles using Artemisia annua L. extract: characterization and application as antibacterial agents
Silver ion bioreduction in nanoparticles using Artemisia annua L. extract: characterization and application as antibacterial agents
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DOI：
10.1186/s13568-020-01002-w
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发表时间：
2020-04-07
期刊：
影响因子：
3.7
通讯作者：
Trchounian, Armen
中科院分区：
文献类型：
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作者：
Aghajanyan, Anush;Gabrielyan, Lilit;Trchounian, Armen
使用具有特定尺寸和形态的植物提取物生物合成金属纳米颗粒是一种简单、无毒且环境友好的方法。本研究主要以青蒿提取物为还原剂和稳定剂合成纳米银（Ag NPs）。 Ag NPs 作为抗菌剂的功能使其进一步用于人类治疗。 pH 和温度对 NP 合成的影响通过紫外吸收光谱进行表征，并通过 410 nm 的表面等离子共振 (SPR) 谱带显示。通过透射电子显微镜（TEM）和动态光散射（DLS）测量纳米颗粒的尺寸和形态。 TEM 图像显示Ag NPs 处于纳米尺寸范围（20-90 nm）并且具有球形形状。我们的研究结果表明，较低浓度（100 μ g mL(-1)）的生物Ag NPs对革兰氏阴性大肠杆菌BW 25113和革兰氏阳性肠球菌海拉ATCC 9790表现出抗菌活性。
The biological synthesis of metal nanoparticles using plant extracts with defined size and morphology is a simple, nontoxic and environmentally friendly method. The present study focused on the synthesis of silver nanoparticles (Ag NPs) by Artemisia annua L. extract as reducing and stabilising agent. The Ag NPs function, as antibacterial agents, is with that they are further used in human therapy. The effects of pH and temperature on the synthesis of NPs were characterized by UV-absorption spectroscopy and shown by surface plasmon resonance (SPR) band at 410 nm. NPs' size and morphology were measured by transmission electron microscopy (TEM) and dynamic light scattering (DLS). TEM images showed that Ag NPs were in a nano-sized range (20-90 nm) and had spherical shape. Our findings demonstrated that lower concentration (100 mu g mL(-1)) of the biogenic Ag NPs exhibited antibacterial activity against Gram-negative Escherichia coli BW 25113 and Gram-positive Enterococcus hirae ATCC 9790.