ANNOTATION - TOTAL AND DIFFERENTIAL LEUKOCYTE COUNT
ANNOTATION - TOTAL AND DIFFERENTIAL LEUKOCYTE COUNT
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DOI：
10.1111/j.1365-2141.1976.tb00966.x
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发表时间：
1976-01-01
影响因子：
6.5
通讯作者：
BAIN, BJ
中科院分区：
文献类型：
--
作者：
ENGLAND, JM;BAIN, BJ
鉴别白细胞计数的两个主要问题是难以获得膜中白细胞的均匀分布和估计各种细胞类型比例的随机误差。白细胞差异计数一般只用于计算各种白细胞的绝对数目。绝对数估计的误差包括鉴别计数的误差和白细胞总数的误差。当白细胞处于悬浮状态时，可以计数到更多的细胞；例如，haemalog D （Technicon公司）对104个细胞进行差异计数。设计了一些简单的技术来实现白细胞计数的自动化。一种这样的系统根据白细胞的体积来区分白细胞，只需要连接到库尔特计数器的脉冲高度分析电路。在测量正常值时，重要的是要考虑不同受试者之间的差异以及每个受试者内部的差异。血液学值受年龄、性别、种族、怀孕、吸烟和口服避孕药的影响。轻微的感染、过敏和运动也会影响白细胞计数，昼夜和月经波动也会影响白细胞计数。
Two main problems with differential leukocyte counting are the difficulty of obtaining an even distribution of leukocytes in the film and the random error in estimating the proportion of the various cell types. The differential leukocyte count is generally used only to calculate the absolute numbers of various leukocytes. The errors in the estimates of the absolute numbers include the errors in the differential count and the errors in the WBC [total leukocyte count]. Many more cells can be counted when the leukocytes are in suspension; the Hemalog D (Technicon Corporation), for example, performs a differential count on 104 cells. Some simple techniques were devised for automating the differential leukocyte count. One such system differentiates leukocytes on the basis of their volumes and requires only pulse height analysis circuits connected to a Coulter Counter. When measuring normal values it is important to consider variations between different subjects as well as variations within each subject. Hematological values are influenced by age, sex, race, pregnancy, smoking and oral contraceptives. Minor infections, allergies and exercise can also affect the leukocyte count and so do diurnal and menstrual fluctuations.