Regulation of transepithelial ion transport in the rat late distal colon by the sympathetic nervous system.
Regulation of transepithelial ion transport in the rat late distal colon by the sympathetic nervous system.
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DOI：
10.33549/physiolres.932795
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发表时间：
2015
影响因子：
2.1
通讯作者：
X. Zhang;Y. Li;X. Zhang;Z. Duan;J. Zhu
中科院分区：
文献类型：
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作者：
X. Zhang;Y. Li;X. Zhang;Z. Duan;J. Zhu
结直肠（晚期远端结肠）受交感神经系统支配，许多结直肠疾病与交感神经系统紊乱有关。结肠直肠离子转运的交感神经调节很少报道。本研究旨在探讨去甲肾上腺素（NE）在正常和儿茶酚胺缺乏状态下的作用，以阐明交感肾上腺素能系统在大鼠结肠直肠离子转运中的调节作用。采用短路电流（I（sc））记录法检测NE诱导的大鼠结肠直肠离子转运;实时荧光定量PCR和Western blotting法检测β-肾上腺素受体和NE转运体（NET）的表达。当内源性儿茶酚胺被利血平耗尽时，结肠直肠的基线I（sc）与对照组相比显著增加。去甲肾上腺素诱发的结肠直肠向下Δ I（sc）是对照组的1.8倍。β 2肾上腺素能受体蛋白在大肠粘膜中的表达高于对照组，但mRNA水平降低。然而，NET的表达显着降低，在儿茶酚胺耗尽大鼠相比，控制。总之，交感神经系统在调节结直肠基础离子转运中起重要作用。交感神经递质紊乱导致离子转运异常，β-肾上腺素受体和NET参与了这一过程。
The colorectum (late distal colon) is innervated by the sympathetic nervous system, and many colorectal diseases are related to disorders of the sympathetic nervous system. The sympathetic regulation of colorectal ion transport is rarely reported. The present study aims to investigate the effect of norepinephrine (NE) in the normal and catecholamine-depleted condition to clarify the regulation of the sympathetic adrenergic system in ion transport in the rat colorectum. NE-induced ion transport in the rats colorectum was measured by short-circuit current (I(sc)) recording; the expression of beta-adrenoceptors and NE transporter (NET) were quantified by real-time PCR, and western blotting. When the endogenous catecholamine was depleted by reserpine, the baseline I(sc) in the colorectum was increased significantly comparing to controls. NE evoked downward deltaI(sc) in colorectum of treated rats was 1.8-fold of controls. The expression of beta(2)-adrenoceptor protein in the colorectal mucosa was greater than the control, though the mRNA level was reduced. However, NET expression was significantly lower in catecholamine-depleted rats compared to the controls. In conclusion, the sympathetic nervous system plays an important role in regulating basal ion transport in the colorectum. Disorders of sympathetic neurotransmitters result in abnormal ion transport, beta-adrenoceptor and NET are involved in the process.