XACT-seq: A photocrosslinking-based technique for detection of the RNA polymerase active-center position relative to DNA in Escherichia coli.
XACT-seq: A photocrosslinking-based technique for detection of the RNA polymerase active-center position relative to DNA in Escherichia coli.
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DOI：
10.1016/j.xpro.2021.100858
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发表时间：
2021-12-17
期刊：
影响因子：
--
通讯作者：
Nickels BE
中科院分区：
文献类型：
--
作者：
Pukhrambam C;Vvedenskaya IO;Nickels BE
XACT-SEQ是一种高通量、单核苷酸分辨率的RNA聚合酶(RNAP)活性中心位置相对于DNA模板的定位技术。Xact-seq克服了依赖于对RNA3‘端的分析(例如，自然延长的转录本测序)或以低分辨率报告RNAP位置的方法(例如，Chip-seq和Chip-exo)的局限性。XACT-seq可用于定位转录起始复合体、初始转录复合体和转录延伸复合体中的RNAP活性中心位置。有关本议定书的使用和执行的完整细节，请参阅。核糖核酸聚合酶活性中心相对于脱氧核糖核酸的定位方法可用于体内411(∼4,000,000)序列文库的转录起始、初始转录和延伸的活性中心定位。Xact-seq克服了依赖于对RNA3‘端的分析(例如，自然延长的转录本测序)或以低分辨率报告RNAP位置的方法(例如，Chip-seq和Chip-exo)的局限性。XACT-seq可用于定位转录起始复合体、初始转录复合体和转录延伸复合体中的RNAP活性中心位置。
XACT-seq (“crosslink between active-center and template sequencing”) is a technique for high-throughput, single-nucleotide resolution mapping of RNA polymerase (RNAP) active-center positions relative to the DNA template. XACT-seq overcomes limitations of approaches that rely on analysis of the RNA 3′ end (e.g., native elongating transcript sequencing) or that report RNAP positions with low resolution (e.g., ChIP-seq and ChIP-exo). XACT-seq can be used to map RNAP active-center positions in transcription initiation complexes, initially transcribing complexes, and transcription elongation complexes. For complete details on the use and execution of this protocol, please refer to. Protocol for mapping of RNA polymerase (RNAP) active-center position relative to DNA Can be applied to transcription initiation, initial transcription, and elongation RNAP active center positions for a library of 411 (∼4,000,000) sequences in vivo XACT-seq (“crosslink between active center and template sequencing”) is a technique for high-throughput, single-nucleotide resolution mapping of RNA polymerase (RNAP) active center positions relative to the DNA template. XACT-seq overcomes limitations of approaches that rely on analysis of the RNA 3′ end (e.g., native elongating transcript sequencing) or that report RNAP positions with low resolution (e.g., ChIP-seq and ChIP-exo). XACT-seq can be used to map RNAP active center positions in transcription initiation complexes, initially transcribing complexes, and transcription elongation complexes.
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影响因子：
16.8
作者：
Chen, Hung-Ta;Warfield, Linda;Hahn, Steven
通讯作者：
Hahn, Steven
影响因子：
14.9
作者：
ODELBERG, SJ;WEISS, RB;WHITE, R
通讯作者：
WHITE, R
影响因子：
7.7
作者：
Yu, Libing;Winkelman, Jared T.;Ebright, Richard H.
通讯作者：
Ebright, Richard H.
DOI：
10.1073/pnas.172226299
发表时间：
2002-08-20
影响因子：
11.1
作者：
Chin, JW;Martin, AB;Schultz, PG
通讯作者：
Schultz, PG
影响因子：
48
作者：
Hino, N;Okazaki, Y;Yokoyama, S
通讯作者：
Yokoyama, S