Murine cytomegalovirus genomic material in marrow cells: relation to altered leukocyte counts during sublethal infection of mice.
Murine cytomegalovirus genomic material in marrow cells: relation to altered leukocyte counts during sublethal infection of mice.
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骨髓细胞中的鼠巨细胞病毒基因组物质：与小鼠亚致死感染期间白细胞计数改变的关系。
DOI：
10.1093/infdis/155.2.207
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Koszinowski,U
中科院分区：
文献类型：
--
作者：
BaleJr,JF;O'Neil,ME;Giller,R;Perlman,S;Koszinowski,U
为了解小鼠巨细胞病毒(MCMV)对造血细胞的感染情况，采用原位杂交技术检测了3周龄小鼠在亚致死性感染过程中骨髓细胞中的MCMV基因组物质。MCMV基因组物质在第3、5、7和14天出现在股骨骨髓细胞中，MCMV基因组的检测与感染性病毒的恢复密切相关。感染高峰出现在第5天，0.003%~0.185%的骨髓细胞含有巨细胞病毒基因组物质。在感染高峰时，MCMV基因组物质主要在骨髓单个核细胞中观察到。在骨髓感染的同时，小鼠出现了显著的白细胞减少，而骨髓细胞中MCMV复制的停止与白细胞增多和恢复正常外周血白细胞计数有关。
To investigate infection of hematopoietic cells by murine cytomegalovirus (MCMV), we used in situ hybridization to detect MCMV genomic material in marrow cells during sublethal infection of three-week-old mice. MCMV genomic material was present in femoral marrow cells on days 3, 5, 7, and 14, and detection of MCMV genomes correlated strongly with recovery of infectious virus. Peak infection occurred on day 5, when 0.003%–0.185% of the marrow cells contained MCMV genomic material. At peak infection, MCMV genomic material was observed predominantly in marrow mononuclear cells. Coincident with marrow infection, mice experienced significant leukopenia, whereas cessation of MCMV replication in marrow cells was associated with leukocytosis and restoration of normal peripheral blood leukocyte counts.