Expansion of banana (Musa acuminata) gene families involved in ethylene biosynthesis and signalling after lineage-specific whole-genome duplications
Expansion of banana (Musa acuminata) gene families involved in ethylene biosynthesis and signalling after lineage-specific whole-genome duplications
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DOI：
10.1111/nph.12710
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发表时间：
2014-05-01
期刊：
影响因子：
9.4
通讯作者：
Yahiaoui, Nabila
中科院分区：
文献类型：
--
作者：
Jourda, Cyril;Cardi, Celine;Yahiaoui, Nabila
全基因组重复 (WGD) 在植物中广泛存在，香蕉 (Musa acuminata) 基因组中出现了三个谱系特异性的 WGD。在这里，我们分析了全基因组测序对涉及乙烯生物合成和信号传导的香蕉基因家族进化的影响，乙烯生物合成和信号传导是香蕉果实成熟的关键途径。使用比较基因组学方法鉴定了香蕉乙烯途径基因，并分析了它们的复制模式和表达谱。 10个香蕉乙烯基因家族中有7个是通过WGD进化的，其中4个（1-氨基环丙烷-1-羧酸合酶（ACS）、乙烯不敏感的3-like（EIL）、乙烯不敏感的3-结合F-box（EBF）和乙烯反应因子（ERF））被优先保留。 AtEIN3 和 AtEIL1（拟南芥乙烯信号传导的两个主要基因）的香蕉直系同源物得到了特别扩展。这种扩展与负责控制 EIL 蛋白水平的 EBF 基因的扩展同时进行。香蕉果实中的基因表达谱表明来自 WGD 的几个 MaEBF 和 MaEIL 基因的功能冗余以及其中一些基因的亚功能化。我们建议香蕉中 WGD 后共同保留 EIL 和 EBF 基因，以维持 EIL 蛋白水平的平衡控制，从而避免组成型乙烯信号传导的有害影响。在进化过程中，亚功能化有利于促进乙烯信号传导的更精细控制。
Whole-genome duplications (WGDs) are widespread in plants, and three lineage-specific WGDs occurred in the banana (Musa acuminata) genome. Here, we analysed the impact of WGDs on the evolution of banana gene families involved in ethylene biosynthesis and signalling, a key pathway for banana fruit ripening. Banana ethylene pathway genes were identified using comparative genomics approaches and their duplication modes and expression profiles were analysed. Seven out of 10 banana ethylene gene families evolved through WGD and four of them (1-aminocyclopropane-1-carboxylate synthase (ACS), ethylene-insensitive 3-like (EIL), ethylene-insensitive 3-binding F-box (EBF) and ethylene response factor (ERF)) were preferentially retained. Banana orthologues of AtEIN3 and AtEIL1, two major genes for ethylene signalling in Arabidopsis, were particularly expanded. This expansion was paralleled by that of EBF genes which are responsible for control of EIL protein levels. Gene expression profiles in banana fruits suggested functional redundancy for several MaEBF and MaEIL genes derived from WGD and subfunctionalization for some of them. We propose that EIL and EBF genes were co-retained after WGD in banana to maintain balanced control of EIL protein levels and thus avoid detrimental effects of constitutive ethylene signalling. In the course of evolution, subfunctionalization was favoured to promote finer control of ethylene signalling.