Human acute leukemia cell line with the t(4;11) chromosomal rearrangement exhibits B lineage and monocytic characteristics.
Human acute leukemia cell line with the t(4;11) chromosomal rearrangement exhibits B lineage and monocytic characteristics.
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DOI：
10.1182/blood.v65.1.21.bloodjournal65121
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发表时间：
1985
期刊：
影响因子：
20.3
通讯作者：
R. Stong;S. Korsmeyer;J. Parkin;D. Arthur;J. Kersey
中科院分区：
文献类型：
--
作者：
R. Stong;S. Korsmeyer;J. Parkin;D. Arthur;J. Kersey
从一个急性白血病复发患者的骨髓中建立了一个细胞系，命名为RS 4;11，该患者的特征是t（4;11）染色体异常。该细胞系和患者的新鲜白血病细胞都具有t（4;11）（q21;q23）和7号长臂的等染色体。形态学上，所有细胞的外观淋巴样。超微结构和细胞化学，约30%的细胞具有髓样特征。细胞末端脱氧核苷酸转移酶呈强阳性。它们为HLA-DR阳性，并表达B谱系细胞的特征性表面抗原，包括通过抗B4、BA-1、BA-2和PI 153/3检测到的那些。免疫球蛋白基因分析显示重链和κ链基因重排。这些细胞缺乏常见的急性淋巴细胞白血病抗原和T细胞系细胞的抗原标志物。细胞与髓样抗体1G 10反应，但不与其他髓样单克隆抗体反应。用12-O-十四烷酰基-佛波醇-13-乙酸酯处理诱导单核细胞样表型，通过细胞化学、功能、免疫学和电子显微镜研究证明。早期淋巴细胞和早期骨髓细胞的标志物的表达代表了一种不寻常的表型，并表明RS 4;11代表了具有双谱系能力的细胞。据我们所知，RS 4;11是第一个建立的t（4;11）相关急性白血病细胞系。
A cell line, designated RS4;11, was established from the bone marrow of a patient in relapse with an acute leukemia that was characterized by the t(4;11) chromosomal abnormality. The cell line and the patient's fresh leukemic cells both had the t(4;11)(q21;q23) and an isochromosome for the long arm of No. 7. Morphologically, all cells were lymphoid in appearance. Ultrastructurally and cytochemically, approximately 30% of the cells possessed myeloid features. The cells were strongly positive for terminal deoxynucleotidyl transferase. They were HLA-DR positive and expressed surface antigens characteristic for B lineage cells, including those detected by anti-B4, BA-1, BA-2, and PI153/3. Immunoglobulin gene analysis revealed rearrangements of the heavy chain and kappa chain genes. The cells lacked the common acute lymphoblastic leukemia antigen and antigenic markers characteristic of T lineage cells. The cells reacted with the myeloid antibody 1G10 but not with other myeloid monoclonal antibodies. Treatment with 12-O-tetradecanoyl-phorbol-13-acetate induced a monocyte-like phenotype demonstrated by cytochemical, functional, immunologic, and electron microscopic studies. The expression of markers of both early lymphoid and early myeloid cells represents an unusual phenotype and suggests that RS4;11 represents a cell with dual lineage capabilities. To our knowledge, RS4;11 is the first cell line established from t(4;11)-associated acute leukemia.