SCANNING FLUORESCENCE CORRELATION SPECTROSCOPY .1. THEORY AND SIMULATION OF AGGREGATION MEASUREMENTS
SCANNING FLUORESCENCE CORRELATION SPECTROSCOPY .1. THEORY AND SIMULATION OF AGGREGATION MEASUREMENTS
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DOI：
10.1016/s0006-3495(86)83709-2
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发表时间：
1986-04-01
影响因子：
3.4
通讯作者：
PETERSEN, NO
中科院分区：
文献类型：
--
作者：
PETERSEN, NO
扫描荧光相关光谱 (S-FCS) 是荧光相关光谱 (FCS) 的改进版，用于测量扩散或流动缓慢的系统（例如生物细胞膜）中的聚集。本文讨论了解释 S-FCS 测量的理论框架，重点讨论了样本大小和形状带来的限制。实验的计算机模拟展示了该技术的潜力，并说明了如何克服一些限制。
Scanning Fluorescence Correlation Spectroscopy (S-FCS) is introduced as an adaptation of Fluorescence Correlation Spectroscopy (FCS) to measure aggregation in systems, such as biological cell membranes, where diffusion or flow is slow. The theoretical framework for interpretation of S-FCS measurements are discussed in this paper with emphasis on the limitations arising from the sample size and shape. Computer simulations of the experiment demonstrate the potential of the technique and illustrate how some of the limitations may be overcome.