The cell lineage of a polyclad turbellarian embryo reveals close similarity to coelomate spiralians
The cell lineage of a polyclad turbellarian embryo reveals close similarity to coelomate spiralians
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DOI：
10.1006/dbio.1998.9084
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发表时间：
1998-12-01
影响因子：
2.7
通讯作者：
Martindale, MQ
中科院分区：
文献类型：
--
作者：
Boyer, BC;Henry, JJ;Martindale, MQ
最近的分子证据表明，Turbellarian扁形动物可能代表现存的基本成员的螺旋，因此可能表现出古老的螺旋发育程序的功能。刻板的四重螺旋卵裂模式的polyclad Turbellarian胚胎，除其他功能外，表明这组可能是密切相关的祖先扁形虫，然而，polyclad胚胎一直是少数实验研究的主题。在这里，我们报告的结果的细胞谱系分析的胚胎的polyclad Hoploplana inquilina的基础上显微注射到卵裂阶段的卵裂球后形成的四个四分体的micromeres。第一个四重奏产生了大部分的侧面和前外胚层的苗勒氏幼虫;第二个四重奏形式主要是背侧和腹侧外胚层以及环形肌肉;第三个四重奏形式只有小克隆的外胚层;和只有第四个四重奏的4d细胞有助于幼虫的结构，形成纵向肌肉，间充质，并可能内胚层。我们的结果表明，多包层胚胎和高等螺旋胚胎的细胞谱系之间存在惊人的相似性，其中四个象限也与幼虫轴具有相同的关系，并产生了可比的幼虫结构，包括从外胚层（2b）和内胚层衍生出中胚层前体（4d）。(C)北京：科学出版社.
Recent molecular evidence suggests the turbellarian Platyhelminthes may represent the extant basal members of the Spiralia and therefore probably exhibit ancient features of the spiralian developmental program. The stereotypic quartet spiral cleavage pattern of the polyclad turbellarian embryo, among other features, indicates that this group may be closely related to the ancestral flatworm; however, polyclad embryos have been the subject of few experimental studies. Here we report the results of a cell lineage analysis of the embryo of the polyclad Hoploplana inquilina based on microinjection of DiI into cleavage-stage blastomeres following formation of each of the four quartets of micromeres. The first quartet gives rise to most of the lateral and anterior ectoderm of the Muller's larva; the second quartet forms largely dorsal and ventral ectoderm as well as the circular muscles; the third quartet forms only small clones of ectoderm; and only the 4d cell of the fourth quartet contributes to larval structure, forming the longitudinal muscles, mesenchyme, and probably endoderm. Our results demonstrate a striking similarity between the cell lineages of polyclad and higher spiralian embryos, in which the four quadrants also bear the same relationships to the larval axes and give rise to comparable larval structures, including derivation of mesoderm from both ectodermal (2b) and endodermal precursors (4d). (C) 1998 Academic Press.