Graphene oxide/poly(N-isopropyl acrylamide)/sodium alginate-based dual responsive composite beads for controlled release characteristics of chemotherapeutic agent
Graphene oxide/poly(N-isopropyl acrylamide)/sodium alginate-based dual responsive composite beads for controlled release characteristics of chemotherapeutic agent
复制标题
DOI：
10.1007/s13726-017-0543-z
复制
发表时间：
2017-07
影响因子：
3.1
通讯作者：
K. Sudhakar;S. J. Moloi;K. Rao
中科院分区：
文献类型：
--
作者：
K. Sudhakar;S. J. Moloi;K. Rao
采用离子凝胶法制备了聚（N-异丙基丙烯酰胺）/海藻酸钠（PNIPAM/NaAlg）复合氧化石墨烯（GO）微球。用傅里叶变换光谱研究了GO与PNIPAM/NaAlg之间的相互作用。X射线衍射分析表明GO在PNIPAM/NaAlg中均匀分布，扫描电镜分析表明GO在PNIPAM/NaAlg中形成了球形复合小球。以5-氟尿嘧啶（5-FU）为模型药物，研究了复合微球的控释特性。发现在所有制剂中的包封效率在90%和92%之间。此外，在1.2和7.4的两种不同pH值以及25和37 °C的不同温度条件下进行珠粒的平衡溶胀比（%）和体外释放研究。结果表明，随着GO浓度的增加，溶胀率降低。在体外释放的研究中进行的pH值和温度的响应，他们证明，5-FU药物从复合珠超过32小时释放，没有爆发释放。细胞毒性结果显示原始复合珠具有良好的细胞相容性。此外，发现与纯5-FU相比，当与复合珠结合时，5-FU的细胞毒性得到改善。因此，可以得出结论，开发的复合珠具有双重响应，并可以用作控制释放载体在癌症药物递送应用。
In this work, graphene oxide (GO)-incorporated composite beads were developed from poly(N-isopropyl acrylamide)/sodium alginate (PNIPAM/NaAlg) using ionotropic gelation technique. The interaction between GO and PNIPAM/NaAlg with Ca2+ions as a cross-linker was investigated by Fourier transform spectroscopy. X-Ray diffraction pattern showed that the GO was distributed uniformly in the PNIPAM/NaAlg with Ca2+ions while scanning electron micrograph technique revealed that composite beads were formed in spherical shape. The controlled release characteristics of composite beads were studied using 5-fluorouracil (5-FU) as anti-cancer model drug. The encapsulation efficiencies were found to be between 90 and 92% in all formulations. Furthermore, the equilibrium swelling ratio (%) and in vitro release studies of the beads were carried out in two different pH values of 1.2 and 7.4 and at different temperature conditions of 25 and 37 °C. The obtained results showed that the swelling ratio decreased with an increase in GO concentration. In vitro release studies performed in response to both pH and temperature and they proved that the 5-FU drug was released from composite beads over 32 h without burst release. Cytotoxicity results showed pristine composite beads are good cytocompatible. In addition, the cytotoxicity of 5-FU was found to be improved when incorporated with composite beads than pure 5-FU. It is therefore concluded that the developed composite beads have dual response and can be used as controlling released carriers in cancer drug delivery applications.