PHENOTYPIC COMPARISON BETWEEN RHIZOSPHERE AND CLINICAL ISOLATES OF BURKHOLDERIA-CEPACIA
PHENOTYPIC COMPARISON BETWEEN RHIZOSPHERE AND CLINICAL ISOLATES OF BURKHOLDERIA-CEPACIA
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DOI：
10.1099/13500872-140-5-1069
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发表时间：
1994-05-01
期刊：
影响因子：
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通讯作者：
VISCA, P
中科院分区：
文献类型：
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作者：
BEVIVINO, A;TABACCHIONI, S;VISCA, P
比较了从根际和临床环境分离的4株洋葱伯克霍尔德菌的表型特征。试验包括最适生长温度、碳源利用、HCN、吲哚-3-乙酸（IAA）和铁载体的产生、蛋白水解活性、固氮作用、对某些植物致病真菌的抑制作用、对人体粘膜和植物根上皮的粘附作用，以及以黄瓜（Cucumis sativus）为实验对象的温室植物生长促进实验。结果表明，从根际分离得到的洋葱芽孢杆菌与临床菌株存在明显差异。从根际分离的菌株在更宽的温度范围内生长，固定氮并产生IAA，不产生蛋白酶，对所研究的植物致病真菌表现出更广泛的抗生素作用，不粘附在人尿路上皮细胞上，促进C. sativus的生长，仅产生羟基酸盐样铁载体。相反，临床分离株不能固定氮或产生IAA，产生蛋白酶，粘附于人尿上皮细胞，不促进C. sativus的生长，除了产生类似羟酸盐的铁载体外，还产生pyochelin和水杨酸铁载体。所有4个分离株均表现出粘附在C. sativus根组织上的能力，并且不能产生HCN。
The phenotypic characteristics of four Burkholderia cepacia strains isolated from the rhizosphere and the clinical environment were compared. Tests included optimum growth temperature, utilization of carbon sources, production of HCN, indole-3-acetic acid (IAA) and siderophores, proteolytic activity, nitrogen fixation, inhibition of some phytopathogenic fungi, adherence to human mucosal and plant root epithelia, and greenhouse-based plant-growth promotion experiments using cucumber (Cucumis sativus). Results indicated that the strains of B. cepacia isolated from the rhizosphere differ markedly from their clinical counterparts. Strains isolated from the rhizosphere grew over a wider temperature range, fixed nitrogen and produced IAA, did not produce proteases, displayed a wider antibiosis against the phytopathogenic fungi studied, did not adhere to human uroepithelial cells, promoted growth of C. sativus and only produced a hydroxamate-like siderophore. In contrast, clinical isolates could not fix nitrogen or produce IAA, produced proteases, adhered to human uroepithelial cells, did not promote the growth of C. sativus and, in addition to a hydroxamate-like siderophore, produced pyochelin and salicylate siderophores. All four isolates exhibited the ability to adhere to the root tissue of C. sativus and were unable to produce HCN.