Hepatic stellate cells (vitamin A-storing cells) change their cytoskeleton structure by extracellular matrix components through a signal transduction system
Hepatic stellate cells (vitamin A-storing cells) change their cytoskeleton structure by extracellular matrix components through a signal transduction system
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肝星状细胞（维生素A储存细胞）通过细胞外基质成分通过信号转导系统改变其细胞骨架结构
DOI：
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发表时间：
1998
影响因子：
2.3
通讯作者：
H. Senoo
中科院分区：
文献类型：
--
作者：
N. Kojima;M. Sato;K. Imai;M. Miura;Y. Matano;H. Senoo
摘要大鼠和人肝星状细胞在聚苯乙烯表面或氨基烷基硅烷覆盖的盖玻片上培养时，表现出扁平的成纤维细胞样形状，具有发育良好的应力纤维。 然而，在I型胶原凝胶上培养细胞导致长的多极细胞突起的伸长，而在Matrigel上培养的细胞保持其圆形。微管和纤维状肌动蛋白的双重荧光染色表明，该过程与胶原纤维一起延伸，并包含微管作为核心，而周边包含纤维状肌动蛋白。黏着斑免疫荧光染色显示，在氨基烷基硅烷包被的盖玻片上培养的细胞，黏着斑主要分布于板状伪足，而在I型胶原凝胶上培养的细胞，黏着斑主要分布于突起的顶端和沿着突起与胶原纤维接触的底面。Wortmannin，以及星形孢菌素和除莠霉素A，抑制伸长过程，并诱导伸长过程的回缩。渥曼青霉素处理也导致从突起到细胞体的粘着斑分布的改变。这些结果表明，细胞表面的整合素结合到间质胶原纤维诱导的过程通过信号事件和随后的细胞骨架组装在肝星状细胞的伸长。
Abstract When cultured on a polystyrene surface or aminoalkylsilane-coated cover glasses, rat and human hepatic stellate cells exhibit a flattened, fibroblast-like shape with well-developed stress fibers. However, culturing the cells on type I collagen gel results in the elongation of long, multipolar cellular processes, whereas cells cultured on Matrigel maintain their round shapes. Dual fluorescence staining of microtubules and fibrillar actin indicated that the processes extend together with collagen fibers and contained microtubules as the core, whereas the periphery contained fibrillar actin. Immunofluorescence staining of vinculin showed that the focal adhesions were distributed mainly in lamellipodia when cultured on aminoalkylsilane-coated cover glasses, whereas in the cells cultured on type I collagen gel they were localized to the tips of the processes and along their bottom surface contacting collagen fibers. Wortmannin, as well as staurosporin and herbimycin A, inhibited the elongation process and induced the retraction of elongated processes. The wortmannin treatment also resulted in an alteration in focal adhesion distribution from the processes to cell bodies. These results indicate that the cell surface integrin binding to interstitial collagen fibers induces the elongation of processes through signaling events and the subsequent cytoskeleton assembly in hepatic stellate cells.
影响因子：
4
作者：
A. Sutton;A. Canfield;S. Schor;M. E. Grant;A. Schor
通讯作者：
A. Sutton;A. Canfield;S. Schor;M. E. Grant;A. Schor